摘要
目的:研究海参脑苷脂(SCC)对非酒精性脂肪肝(NAFLD)大鼠的改善作用。方法:通过在饲料中添加乳清酸复制NAFLD大鼠模型,将大鼠按体重随机分为对照组、模型组、海参脑苷脂低剂量组和海参脑苷脂高剂量组,喂食10 d后观察其血清、肝脏脂质和转氨酶活性的变化,以及肝脏脂肪酸组成变化。结果:相比对照组,模型组血清总胆固醇(TC)和甘油三酯(TG)含量降低(P<0.05),海参脑苷脂可增加血清脂质含量(P<0.05);模型组肝脏TC和TG含量升高显著(P<0.05,P<0.01),不同剂量的海参脑苷脂均可降低肝脏脂质积累(P<0.05,P<0.01);模型组谷丙转氨酶(ALT)和谷草转氨酶(AST)水平升高(P<0.05,P<0.01),不同剂量的海参脑苷脂可改善肝脏损伤水平(P<0.05,P<0.01);另外,模型组肝脏硬脂酰辅酶A去饱和酶(SCD)活性显著升高(P<0.05),海参脑苷脂明显降低该酶活性(P<0.05)。结论:海参脑苷脂可显著改善乳清酸诱导的大鼠脂肪肝,并且呈现明显的剂量效应。
Objective： To investigate the effect of sea cucumber cerebroside（SCC） on the lipid metabolism in rats with orotic acid-induced fatty liver.Methods： The non-alcoholic fatty liver disease（NAFLD） model was established by adding orotic acid to the diets in rats.The rats were randomly assigned to four groups：control group,NAFLD group,NAFLD ＋ low SCC group and NAFLD ＋ high SCC group.After 10 days of feeding,the serum and hepatic lipid concentrations and the aminopherase activities were measured;the composition of hepatic fatty acids was also analyzed.Results： The serum TC and TG levels reduced significantly in the NAFLD group as compared with the controls（P0.05）,while the sea cucumber cereborside feeding raised the serum lipid concentrations（P0.05）.The hepatic TC and TG levels dramatically increased in the NAFLD group in comparison with the controls（P0.05,P0.01）,while the hepatic lipid accumulations decreased in both SCC groups（P0.05,P0.01）.The ALT and AST activities in the NAFLD group increased markedly when compared with the controls（P0.05,P0.01）,while the sea cucumber cerebroside feeding attenuated the hepatic injury levels（P0.05,P0.01）.Compared with the control group,the stearoyl-CoA desaturase（SCD） activity increased significantly in the NAFLD group（P0.05）,but decreased in SCC groups（P0.05）.Conclusion： Sea cucumber cerebroside can attenuate the rat fatty liver induced by orotic acid.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期493-498,共6页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金(30972285)
国家科技支撑计划(2008BAD94B05)
