摘要
目的:研究染料木素(Genistein)对人骨髓瘤细胞株XG-1的核因子-κB(NF-κB)表达的影响及Genistein处理前后XG-1细胞内蛋白激酶B(Akt)和磷酸化Akt的表达。方法:凝胶电泳迁移率试验检测Genistein处理前后NF-κB在XG-1细胞核内的表达水平;蛋白质印迹法检测Genistein处理前后XG-1细胞内Akt和磷酸化Akt的表达。结果:凝胶电泳迁移率实验(EMSA)发现,NF-κB在XG-1细胞株中持续激活,而Genistein能下调这一激活。随着浓度逐渐提高,细胞核内NF-κB的活力逐渐下降,呈剂量依赖,其中溶剂对照组与10mg/L的Genistein组相比,χ2=4.614,P<0.05;溶剂对照组与15mg/L的Genistein组相比,χ2=4.324,P<0.05。Genistein能拮抗这一作用。XG-1细胞用不同浓度Genistein处理24h,可见Akt无明显变化,而磷酸化Akt随着浓度逐渐提高,表达逐渐下降,呈剂量依赖,其中溶剂对照组与10mg/L的Genistein组相比,χ2=4.751,P<0.05;溶剂对照组与15mg/L的Genistein组相比,χ2=5.114,P<0.05。结论:NF-κB在XG-1人类骨髓瘤细胞株中持续激活,Genistein下调NF-κB的激活形式而且抑制了Akt磷酸化,Genistein抑制细胞的NF-κB的激活部分经由Akt通路完成。
OBJECTIVE：To study the effect of Genistein on NF-κB expression of XG-1 multiple myeloma cells,and examine the effect of Genistein on expressions of the Akt and phospho-Akt in XG-1 cells. METHODS：XG-1 multiple myeloma cells were treated with different concentrations of Genistein. The NF-κB in the cells was indicated by electrophoretic mobility gel shift assay. The expressions of Akt and phospho-Akt in XG-1 cells were detected by Western blot assay. RESULTS：NF-κB was constitutively active in XG-1 myeloma cell lines,and Genistein could down-regulate it as indicated by electrophoretic mobility gel shift assay. A significant difference between 10 mg/L （χ2=4.614,P0.05）,15 mg/L （χ2=4.324,P0.05） Genistein and the control was found. XG-1 myeloma cells showed consitutively active Akt and Phospho-Akt. Genistein suppressed Akt phosphorylation but not Akt. A significant difference between 10 mg/L （χ2=4.751,P0.05）,15 mg/L（χ2=5.114,P0.05） Genistein and the control was found. CONCLUSION：NF-κB is constitutively active in XG-1 human myeloma cell lines examines and Genistein can down-regulates NF-κB by suppressing the constitutive Akt phosphorylation.
出处
《中华肿瘤防治杂志》
CAS
2010年第17期1341-1343,1354,共4页
Chinese Journal of Cancer Prevention and Treatment
