摘要
目的检测结直肠癌组织中E-钙粘素的表达及其淋巴结微转移，探讨两者间的关系。方法以CK20mRNA为标记物，应用RT-PCR对60例结直肠癌根治性手术切除的淋巴结标本共782例进行检测；免疫组化法（SP法）检测结直肠癌组织、癌旁组织、淋巴结微转移及正常对照组中E-钙粘素的表达。通过二者的综合分析，试图发现抑癌基因E-钙粘素在结直肠癌淋巴结微转移的生物学行为中的作用。结果CK20法检出45例（75.0％）患者有淋巴结转移，阳性淋巴结总数为436个（55．8％）；常规病理学检出34例（56．7％）患者有淋巴结转移，检出阳性淋巴结数为356个（45．5％）；两组比较，差异具有统计学意义（x2=16．72，P〈0．05）。11例检出淋巴结微转移患者癌组织中6例（54．5％）E-Cadherin表达阴性，而15例未检出淋巴结微转移癌组织中仅有1例（6．67％）表达阴性，两者比较差异有统计学意义（x2=4．160，P=0．041）。结论E—Cadherin的表达减弱或消失参与了结直肠癌淋巴结微转移的发生。
[ Abstract] Objective Purpose To detect the expression of E-cadherin in colorectal carcinoma and lymph node micrometastases, and investigate their relationship. Methods 82 cases of lymph node simple was decteeted with RT-PCR method using CK20 mRNA acridinium ester, in screening coloreetal carcinoma lymph node micrometastases. E-Cadherin expression analysed by immuno-histochemieal staining （the SP method） in eoloreetal cancer tissue, tissue nearby cancer, the normal control tissue. Results In 60 cases of colorectal ear-cinoma,the expression of E-Cadherin was 71.67%. Number of positive nodes was 55.8%, the expression of E- cadherin was 56. 7% （34 cases） and number of positive nodes was 45.5% （356 cases） through routine pathological examination. The low level expression of E-Cadherin was positively correlated with the Dukes staging, colorectal carcmoma （X 2=16. 72, P 〈 0.05 ） The expresserosa infiltration, lymph node and liver metastasis of colorectal carcinoma（x2 =16.72,P〈0.05）.The expression of E-Cadherin was negative in 6 of the 11 patients with lymph node micrometastases, and in 6. 67% 1 of the rmcrometastases（x 2=4. 160 ,P = 0.041）. Conclusion The negative expression of E-Cadherin may contribute to lymph node micrometastases in colorectal carcinoma.
出处
《中国临床实用医学》
2010年第10期6-8,共3页
China Clinical Practical Medicine
基金
基金项目：山东省烟台市科技发展计划资助项目（项目编号：2006131-11）
