摘要
目的:明确在乙、丁型肝炎病毒重叠感染时HDV对HBV复制和HBVM表达有无抑制作用。方法:HBV DNA定量采用聚合酶链反应法,抗HD IgM采用CIA抗体捕获法,抗HD采用EIA竞争法,HDAg采用EIA夹心法,HBVM采用酶联免疫吸附法。结果:重叠感染组和单纯乙肝组的HBV DNA定量高滴度(≥10~6cps/ml)病例数比率分别为75％和67.74％,各型肝炎(慢性肝炎、肝硬变、慢性重型肝炎)HBV DNA定量高滴度病例数比率,重叠感染分别为68.42％、71.43％、100％,单纯乙肝组分别为65.38%、66.67％、100％。两组病例HBVM主要表达模式均为HBsAg(+)、HBeAg(+)、抗HBc(+)或HBsAg(+)、抗HBe(+)、抗HBc(+),重叠感染组分别为57.14％、17.86％,单纯乙肝组分别为54.84％、16.13％,两组主要表达模式的HBV DNA定量高滴度病例数比半,重叠感染组分别为87.5％、60％,单纯乙肝组分别为88.24％、60％,均无显著性差异(P>0.05)。结论:HDV对HBV的复制和HBVM表达无明显抑制作用。
Aim: To define whether HDV can inhibit replication of HBV and expressin of HBVM in hepatitis B and D superinfection or not. Methods: HBV DNA quantity was detected by PCR using fluorescent quantitative analysis system. Anti-HDIgM, Anti-HD and HDAg were detected by ELISA. HBVM was detected by EL1SA. Results: Case rates of high titer of HBV DNA (≥106cps/ml) were 75% in superinfection group were 67. 74% in hepatitis B alone group. High titer of HBV DNA in chronic hepatitis, cirrhosis and chronic severe hepatitis were 68.42%. 71. 34% and 100% in superinfection group and 65. 38% . 66.67% and 100% in hepatitis B alone group respectively. Main expreessive profile of two groups were positive for HBsAg. HBeAg . Anti-HBc or positive for HBsAg . Anti-HBe, Anti-HBc. They accounted for 57. 14% . 1 7. 86% respectively in super infect ion group and 54. 84% . 16. 13% respectively in hepatitis B alone group. Case rates of high titer of HBV DNA in two expressive profiles were 87. 5%. 60% in superinfection group respectively and 88.24%, 60% in hepatitis R alone group respectively. There were all no significant difference (P>0. 05). Conclusion: HDV could not inhibit replication of HBV and expression of HBVM.
出处
《中西医结合肝病杂志》
CAS
1999年第3期10-11,共2页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
