摘要
目的观察缺血预处理对骨骼肌缺血再灌注损伤的保护作用。方法选择２４只健康兔，随机等分为实验组和对照组。实验组先进行缺血预处理，再持续阻断后肢血流４ｈ；对照组直接阻断后肢血流４ｈ，制作骨骼肌缺血再灌注损伤模型。测定再灌注期血清中肌酸磷酸激酶（ＣＰＫ）和天门冬氨酸氨基转移酶（ＡＳＴ），镜下观察骨骼肌结构变化。结果实验组血清中ＣＰＫ和ＡＳＴ的含量均明显低于对照组（Ｐ＜００５）。实验组骨骼肌线粒体空泡变性和肌原纤维溶解程度明显轻于对照组。结论缺血预处理能增强骨骼肌对缺血的耐受性，减轻骨骼肌缺血再灌注损伤程度。
Objective To observe the effect of protecting skeletal
muscle from ischemia and reperfusion injury with ischemic preconditioning. Methods 24
healthy rabbits were selected and randomly divided into either experiment or control
group equally.The model of ischemia and reperfusion injury was made by stopping
blood flows of rabbits hind limbs for 4 hours, the experiment preconditioned and the
control undergoing nothing.The contents of creatine phosphate kinase (CPK) and aspartate
aminotransferase (AST) in the serum were measured respectively during reperfusion.
Structural changes of the skeletal muscle were observed with light and electron
microscope. Results The contents of CPK and AST in the experimental group were
lower than that in the control (P<005). Compared with the control, mitochondrial vacuolar
degeneration and dissolution of myofibril in the experimental group were significantly
reduced. Conclusion IPC enhances the continuous ischemic tolerance of skeletal muscle
and reduces its ischemia and reperfusion injury.
出处
《临床骨科杂志》
1999年第2期85-87,共3页
Journal of Clinical Orthopaedics
关键词
再灌注损伤
预处理
骨骼肌缺血
reperfusion injury
muscle,
skeletal
ischemic preconditioning
aspartate aminotransferase
creatine kinase
rabbit
