摘要
目的:观察长时间体外膜肺氧合(ECMO)过程中犬凝血系统的变化规律。方法:选择健康杂种犬6只,进行ECMO转流36h,并于实验开始前30min、开始后6h、12h、24h、36h分别取静脉血10mL,动态检测PT、APTT、TT值来研究实验动物凝血系统的变化规律,检测PLT以及GMP-140值来研究实验动物血小板的动态变化过程。结果:实验开始后6h凝血系统处于稳定状态,ECMO转流12h后凝血系统将被激活,同时出现血小板脱颗粒反应,24h、36h更为明显。结论:ECMO转流12h后凝血系统将被激活同时出现PLT脱颗粒反应,故12h后应采取干预措施。
Objective：To explore the variation of coagulation system during prolonged extracorporeal membrane oxygenation（ECMO） of dogs.Methods：6 hybrid dogs in good condition were performed femoral AV ecmo mode for 36h.Venous blood sample were taken at the following time point：30 min before the operation,6 h,12 h,24 h and 36 h after the operation.Levels of APTT、PT and TT were measured to explore the variation of coagulation system of experimental animals.Levels of PLT、GMP-140 were measured to explore the dynamic variation of blood platelet of experimental animals.Results：The coagulation system of experimental dogs were not activated in the first 6 h during operation.After running 12 h,the coagulation system was activated,simultaneously,the degranulation reaction of platelet was observed,and the reaction was especially obvious after running 24 h,36 h.Conclusion：The coagulation system was activated,at the same time,the PLT degranulation reaction began to occurs,after running ECMO 12 h.Therefore,measures should be taken to protect the coagulation system at this time.
出处
《心肺血管病杂志》
CAS
2010年第5期415-417,共3页
Journal of Cardiovascular and Pulmonary Diseases
基金
天津市科技发展重点攻关项目(06YFSZSF01500)
关键词
体外膜肺氧合
凝血系统
干预
动物实验
犬
Extracorporeal membrane oxygenation
Coagulation system
Intervention
Animals laboratorg
dog
