摘要
目的探索青蒿琥酯对狼疮模型鼠-MRL/lpr鼠狼疮性肾炎(LN)的疗效及作用机制。方法分别以青蒿琥酯50mg/(kg.d)和相同体积生理盐水灌胃治疗MRL/lpr鼠,治疗16周后检测小鼠24 h蛋白尿、血肌酐水平,检测小鼠外周血血清中ICAM-1浓度及淋巴细胞表面CD54阳性率,检测小鼠肾组织中ICAM-1表达。结果青蒿琥酯能够显著减少狼疮鼠的蛋白尿(P<0.01)及血肌酐水平(P<0.05),显著降低狼疮鼠的血清中可溶性ICAM-1浓度(P<0.05)及淋巴细胞表面CD54的阳性率(P<0.05),导致肾组织中ICAM-1的表达明显下降。结论青蒿琥酯显著缓解MRL/lpr鼠LN病情,此作用可能与抑制ICAM-1的表达有关。
Objective To investigate the curative effect and mechanism of artesunate on nephritis（LN） in MRL/lpr mice.Methods MRL/lpr mice were treated by artesunate（50 mg/kg·d） and the same volume of physiological saline for 16weeks respectively.24 hours urine protein,serum creatinine, serum ICAM-1 concentration,the expression of CD54 on peripheral blood lymphocytes,and the expression of ICAM-1 in kidney tissue were measured.Results Urine protein,serum creatinine,serum ICAM-1 concentration,the percentage of CD54 positive lymphocytes and the expression of ICAM-1 in kidney were all significantly decreased by artesunate.Conclusion Artesunate obviously relieves LN in MRL/lpr mice.This effect is correlated with its inhibition on ICAM-1 expression.
出处
《实用临床医药杂志》
CAS
2010年第9期1-3,共3页
Journal of Clinical Medicine in Practice
基金
中华医学会风湿病学分会研究与发展基金项目(08010180096)
江苏省卫生厅科技项目(H200878)
江苏省南京市医学科技发展项目(YKK07064)
