摘要
目的研究硒化壳聚糖(Sc)对人急性早幼粒白血病NB4细胞分化的影响,探讨这种作用与细胞CD11b和c-fos抗原表达的关系。方法四甲基偶氮唑蓝(MTT)法检测细胞增殖;硝基蓝四氮唑(NBT)还原法检测细胞分化;流式细胞术检测细胞CD11b和c-fos抗原表达。结果 50～200mg/LSc对NB4细胞增殖有抑制作用,呈时间剂量依赖趋势;经50mg/LSc作用48h后,NBT阳性细胞数明显增多,CD11b及c-fos抗原的表达较对照组明显增强[(55.4±3.2)%与(2.1±1.3)%,(42.3±3.7)%与(12.7±1.6)%,P<0.01)],而100mg/L及以上浓度Sc组CD11b及c-fos抗原表达则逐渐减小。结论 Sc可通过上调NB4细胞CD11b及c-fos抗原表达来促进细胞分化。
Objective To study the effect of selenium chitosan（Sc） on differentiation of NB4 cells cultured in vitro and explore its relationship with the CD11b and c-fos antigen molecules. Methods The differentiation and proliferation effect of Sc was measured by NBT reduced method and MTT method,respectively; FCM was used to examine the expression of CD11b and c-fos antigen molecules in tumor cells. Results 50 mg/L to 200 mg/L of Sc could inhibit cell proliferation in a dose and time dependent manner. An exposure of NB4 cells to 50 mg/L of Sc for 48 h could increase NBT reduced positive cells. At the same time the abundance of CD11b and c-fos were strongly up-regulated [（55.4±3.2）% vs （2.1±1.3）%,（42.3±3.7）% vs （12.7±1.6）%,P0.01）]. However,the value of CD11b and c-fos were decreased in NB4 cells treated with 100 mg/L to 200 mg/L of Sc. Conclusion Sc could promote NB4 cells differentiation by up-regulating the abundance of CD11b and c-fos antigen molecules.
基金
湖北省教育厅项目(B200624004)
湖北省十堰市科学技术局项目(十科通[2006]18号)
