摘要
最近的研究发现：ＡｃＮＰＶ的ｖｐ３９基因与侵染密切相关［１］．在侵染过程中，ＶＰ３９蛋白与宿主的肌动蛋白结合，使其重排形成缆索（ｃａｂｌｅ）．导致细胞骨架发生变化有利于病毒编码的蛋白酶的水解．最后，子代病毒颗粒大量形成，宿主昆虫体全部液化成为脓水．可...
Autographa californica nuclear polyhedrosis virus (AcNPV)DNA as a template was amplified successfully by PCR technique using forward and reverse primers synthesized from AcNPV vp 39 gene.The PCR fragment was inserted into pGEM 3zf(+) plasmid DNA.It was demonstrated that the amplified 1 3 kb fragment is the nuclear capsid protien gene ( vp 39) by sequencing of 5′ and 3′ terminal regions.The AcNPV vp 39 gene was inserted into the expression vector pRSET A,and highly expressed by IPTG induction in E.coli BL21.SDS PAGE analysis showed that the expressed protein was about 38 kD, and the expressed amount reached maxium in 4 h with IPTG induction.The immediately early gene IE1 promoter of AcNPV was subcloned into pGEM Ac39 to get the expression vector pAcIE1 39 in order to drive the vp 39 gene for transient expression in insect cells.Since the pAcIE1 39 was transfected or co transfected with LacZ AcNPV DNA into Sf9 cell,the insect cells were disrupted and proliferation of the AcNPV was reduced.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第2期340-343,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金
国家教委博士点基金
关键词
苜蓿银纹夜蛾
核型多角体病毒
基因克隆
细胞
Nuclear capsid protein gene ( vp 39)
PCR amplification
Transient expression
AcNPV
Cell structure
