摘要
目的观察表没食子儿茶素没食子酸酯（epigallocatechingallate,EGCG）对大鼠血管平滑肌细胞钙化的影响。方法制备β-甘油磷酸盐诱导血管平滑肌细胞（vascular smooth muscle cells,VSMCs）钙化模型。实验分为4组：对照组、钙化组、单纯EGCG组及EGCG干预组（又分为10-7、10-6、10-5和10-4mol.L-1的EGCG 4个亚组）。Von Kossa染色观察细胞钙化情况,生化试剂盒比色法测定钙含量及碱性磷酸酶（alkalinephosphatase,ALP）活性。结果 VSMCs钙化组Von Kossa染色见平滑肌细胞内和细胞间基质有大量黑色颗粒聚集;与对照组比较,钙化组VSMCs钙含量、ALP活性均有明显增加;10-7~10-4mol.L-1EGCG干预组VSMCs钙含量、ALP活性呈剂量依赖性有显著降低。结论 EGCG具有抑制VSMCs钙化的作用。
Objective To explore the effects of epigallocatechingallate（EGCG） on vascular calcification.Methods Calcific vascular smooth muscular cells（VSMCs） were induced by β-glycerophosphate in rats.Rats were invided into four groups：the control,the calcification,EGCG and EGCG intervention groups（10-7,10-6,10-5 and 10-4 mol·L-1 subgroups）.Von Kossa staining,the Ca2＋ content and the alkaline phosphatase（ALP） activity in VSMCs were measured.Results Lots of gathered black granules were found among the smooth muscle cells and its matrix by Von Kossa staining in VSMCs calcifacation group;more Calcium contents and ALP activities were observed in calcification group than those in control group;after treated with EGCG 10-7~10-4mol·L-1,the calcification in VSMCs was also attenuated and the total Ca2＋ content and ALP activity in VSMCs showed decrease and a concentration-dependent manner.Conclusion The results indicated that EGCG can significantly attenuate the VSMCs calcification.
出处
《宁夏医科大学学报》
2010年第6期660-662,F0003,共4页
Journal of Ningxia Medical University
基金
宁夏回族自治区卫生厅重点科研计划项目(2008032)
宁夏医科大学面上项目(XM200812)
