摘要
目的探讨姜黄素对3T3-L1脂肪细胞脂联素和抵抗素mRNA表达的影响。方法在诱导3T3-L1前脂肪细胞分化过程中加入姜黄素,于第6 d收集细胞,或用姜黄素处理分化成熟3T3-L1脂肪细胞,24 h后收集细胞,采用逆转录聚合酶链反应(RT-PCR)方法检测脂肪细胞中脂联素和抵抗素mRNA水平。结果在3T3-L1前脂肪细胞分化期间,姜黄素2.5,5,10,15和20μmol/L分别使脂联素mRNA表达增强48.7%,25.6%,89.7%,128.2%和207.7%,抵抗素mRNA表达下降了42.3%,35.4%,31.5%,58.3%,33.9%;20μmol/L姜黄素使成熟脂肪细胞脂联素mRNA表达增强203.3%;5,10,20μmol/L的姜黄素分别使抵抗素mRNA的表达降低15.2%,49.0%和55.6%。结论姜黄素可增强脂肪细胞脂联素mRNA表达,降低抵抗素mRNA表达。
Objective To explore the effect of curcumin on the expressions of adiponectin and resistin mRNA in 3T3-L1 adipocytes,and to analyze possible mechanism of curcumin in the prevention of diabetes at cellcular level.Methods Curcumin at concentrations of 2.5,5,10,15,and 20 μmol/L was added in 3T3-L1 preadipocytes during induced-differentiation for six days and 5,10,and 20μmol/L in mature 3T3-L1 adipocytes for 24 hr,respectively.Then the expressions of adiponectin and resistin mRNA in 3T3-L1 preadipocytes during induced-differentiation and mature 3T3-L1 adipocytes were detected with RT-PCR.Results The expression of adiponectin mRNA increased and resistin mRNA decreased in 3T3-L1 preadipocytes treated with curcumin compared to 3T3-L1 preadipocytes without curcumin during induced-differentiation.The expression of adiponectin mRNA increased and resistin mRNA decreased in mature 3T3-L1 adipocytes treated with curcumin compared to that of in mature 3T3-L1 adipocytes without curcumin.Conclusion The increase of adiponectin expression mRNA and the decrease of resistin mRNA in 3T3-L1 adipocytes may be one of mechanisms of curcumin in the prevention of diabetes.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第10期1248-1250,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30771805)
