摘要
目的:以非离子表面活性剂为载体材料进行斑蝥素囊泡的制备考察,并评价其特性表征。方法:建立了注入法制备斑蝥素非离子表面活性剂泡囊的制备工艺;采用离心法和HPLC法测定斑蝥素囊泡的包封率;考察不同处方因素对囊泡体外表征的影响,在此基础上正交设计优化处方;对最佳处方囊泡的形态学、粒径、Zeta电位等性质进行了考察。结果:注入法获得的CTD-NS的包封率为(27.5±2.0)%,粒径(209.8±0.5)nm,Zeta电位(41.5±0.65)mV。结论:以非离子表面活性剂A和B联用为载体材料,注入法制备斑蝥素非离子表面活性剂囊泡,制备方法简单,性质稳定。
Objective：To study the preparation of cantharidin entrapped non-ionic surfactant vesicle（noisome）and evaluate its quality. Method： The niosome loaded with cantharidin was prepared using injection method by non-ionic surfactants as the carrier. An centrifugation separation method and HPLC analysis method of the cantharidin were established to detect the entrapment efficiency. The optimum preparation technology was established by a orthogonal experiment. The morphology, and particle size were studied to evaluate the preparation. Result： The average size of niosomes were（209.8±0.5）nm.The entrapment efficiency of the CTD-NS was（27.5%±2.0%）and Zeta potential was （41.5±0.65）mV. Conclusion：The preparation of cantharidin noisome by TweenA and SpanB is practicable and successful. These experiments can be the basement of developing targeting drug delivery system.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第19期2546-2550,共5页
China Journal of Chinese Materia Medica
基金
中国中医科学院自主选题项目(ZZ2006118
Z02094)
关键词
斑蝥素
注入法
非离子表面活性剂
囊泡
cantharidin
injection method non-ionic surfactant
niosome
