摘要
[目的]检测贵州省苗族及布依族正常人群细胞色素氧化酶1A1*2C(CYP1A1*2C)基因多态性。[方法]采用Taqman-MGB探针,通过实时定量PCR(real-time PCR)对贵州省三都县125名苗族及122名布依族人群的血液样本进行CYP1A1*2C基因多态性分析,并采用χ2检验比较两民族该基因分布的差异。[结果]CYP1A1*2C野生纯合子(基因型AA)、突变杂合子(基因型AG)、突变纯合子(基因型GG)在苗族及布依族中基因型频率分别为65.6%、28.0%、6.4%及68.9%、25.4%、5.7%;A和G在苗族及布依族中基因频率分别为79.6%、20.4%及81.6%、18.4%,两民族人群的基因多态性分布差异无统计学意义。[结论]中国贵州省苗族及布依族人群中CYP1A1*2C基因型频率分布没有明显不同。
[ Objective] To investigate the gene polymorphisms of CYP1A1＊2C in Chinese Miao, Buyi nationalities. [Methods] Taqman-MGB probe was used in real-time RealTime-PCR to test the polymorphisms of CYP1A1＊2C in Chinese Miao（125）, Buyi（122）people, and the significant difference between Miao and Buyi was checked by χ2 test.[ Results] The distribution of wild homozygote（genetic type AA）, mutant heterozygote（genetic type AG）, mutant homozygote（genetic type GG）of CYP1A1＊2C in Chinese Miao, Buyi populations were 65.6%, 28.0%, 6.4% and 68.9%, 25.4%, 5.7%, respectively. The distribution of A and G allele frequency of CYP1A1＊2C in Miao, Buyi populations were 79.6%, 20.4% and 81.6%, 18.4%, respectively.[ Conclusion] The distribution of genetic frequency and allele gene frequency of CYP1A1＊2CC are without statistically significance in difference between Miao, Buyi nationalities in Guizhou Province China.
出处
《环境与职业医学》
CAS
北大核心
2010年第9期560-562,共3页
Journal of Environmental and Occupational Medicine
