摘要
目的和方法：研究Ｕ４６６１９（９，１１－ｄｉｄｅｏｘｙ－１１α，９α－ｅｐｏｘｙｍｅｔｈａｎｏ－ｐｒｏｓｔａｇｌａｎｄｉｎＦ２α）对培养的人气管平滑肌细胞增殖的作用。分离人的气管平滑肌细胞并且进行传代培养。在培养基中加入各种浓度的Ｕ４６６１９，计数细胞并且测定［３Ｈ］－胸腺嘧啶核苷（［３Ｈ］－ＴｄＲ）掺入量和三磷酸肌醇［Ｉｎｓ（１，４，５）Ｐ３］累积量。结果：Ｕ４６６１９在一定范围内（１ｎｍｏｌ／Ｌ～１００ｎｍｏｌ／Ｌ）以浓度依赖的方式增加人气管平滑肌的细胞数（Ｐ＜００１）。Ｕ４６６１９也增加［３Ｈ］－ＴｄＲ的掺入量和Ｉｎｓ（１，４，５）Ｐ３的累积量（Ｐ＜００１）。磷脂酶Ｃ抑制剂新霉素阻止Ｉｎｓ（１，４，５）Ｐ３累积量的增加（Ｐ＜００１），但对Ｕ４６６１９的有丝分裂原的活性没有作用。结论：显示Ｕ４６６１９刺激培养的人气管平滑肌细胞增殖。
AIM:To study the role of U 46619 in stimulating the proliferation of cultured human airway smooth muscle cell (ASMC).METHODS:Human ASMC were isolated and subcultured, varying concentrations of U 46619 were added to the media. Cell counts were obtained, -thymidine incorporation (-TdR) and inosital 1,4,5-trisphosphate [Ins(1,4,5)P 3] accumulation were measured.RESULTS:U 46619 (1 nmol/L-100 nmol/L) increased cell number in a concentration depend manner ( P <0 01) U 46619 also increased -TdR and (1,4,5)P 3 accumulation ( P <0 01). The latter response was blocked by phospholipase C inhibition with neomycin ( P<0.01 ),however, neomycin had no effect on the promitogenic action of U 46619 .CONCLUSION: U 46619 stimulates the proliferation of cultured human ASMC.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第5期456-458,共3页
Chinese Journal of Pathophysiology
