摘要
目的：从毫秒级功能变化水平实时观察骨骼肌在潜伏期内的细胞超微结构形态变化。方法：采用双向红外线探测器－计算机控制的电刺激与超低温快速冷冻固定同步技术，对电刺激后的蟾蜍腓肠肌组织作快速冷冻固定，采用透射电镜对该肌在电刺激后０．８，５．６，８．４ｍｓ及静息期的超微结构变化进行对比研究。结果：骨骼肌组织处于静息期时，其肌膜与肌纤维的间隙较宽，受电刺激后０．８ｍｓ开始至８．４ｍｓ，肌膜与肌细胞间的间隙大大变窄。并发现骨骼肌组织在电刺激后０．８，５．６及８．４ｍｓ时肌浆网前端的膜产生两个圆孔，而当骨骼肌处于静息期时肌浆网膜并无此形态结构。结论：当骨骼肌兴奋－收缩偶联发生时，肌膜在产生去极化的同时产生位移，并与肌纤维的间距发生改变；骨骼肌收缩时肌浆网膜出现小孔。
Objective:To study the ultrastructural morphological changes at millisecond time course level in skeletal muscle during excitation contraction coupling after electrical stimulation. Methods: Two way infrared detectors with a computer to control the signal of electric stimulation and quick freezing fixation were employed in this test. The ultrastructure of toad skeletal muscle was studied by transmission electronic microscopy after its skeletal muscle was stimulated in 0.8, 5.6, 8.4 ms. The skeletal muscular ultrastructure at known millisecond time course were observed following quick freezing after a single electrical stimulation. Results:The space between sarcolemma and muscle myofibril was strikingly narrowed from 0.8 ms to 8.4 ms after electric stimulation, and 2 holes were observed in the front of sarcoplasmic reticulum membrane at the same time. Conclusion: During excitation contraction coupling, the sarcolemma has positional shaft and space changes with myofibril when depolarization occurs, besides, there are small holes on the sarcolemma when skeletal muscles contract.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第3期151-153,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
骨骼肌
兴奋-收缩偶联
超微结构
skeletal muscle
excitation contraction coupling
ultrastructure
