摘要
采用免疫组织化学S-P法检测41例肾癌、18例癌旁正常肾组织及5例正常肾组织中P16蛋白的表达,以探讨P16蛋白的表达与肾癌组织学类型、临床分期、病理分级及预后的关系。结果发现P16蛋白在肾癌及正常肾组织中的阳性表达率分别为58-5%(24/41)及91-3%(21/23),两组阳性表达差异具有显著性(χ^(2)=7-60,P<0-05);P16蛋白表达在肾透明细胞癌及颗粒细胞癌间无差异,随肾癌分级、分期升高而明显降低,Ⅰ级与Ⅱ、Ⅲ级比较阳性表达具有显著差异,低分期组(Ⅰ、Ⅱ)与高分期组(Ⅲ、Ⅳ)比较具有显著差异;P16阳性表达组术后5年生存率明显高于阴性表达组。结果提示:P16基因失活在肾癌的发生发展中起重要作用,P16基因及蛋白的检测可作为肾癌的辅助诊断及预后判断的参考指标。
Immunohistochemistry detection of P16 expression was carried out in 41 cases of renal carcinoma,in 18 normal renal tissue adjacent to neoplastic lesions and in 5 normal renal tissue.The positive rate of P16 expression was higher in normal renal tissue(91.3%)than in tumor tissue(58.5%)(P<0.05).The positive expression rate in low grade tumors(Ⅰ)was significantly higher than the high grade tumors(Ⅱ、Ⅲ),and the lower the stage of the tumors,the higher the positive rate of P16.There was a significant difference between P16 positive and negative cases in 5 year survival rate,with no significant difference between clear renal cell carcinoma and granular cell carcinona.The results suggested that P16 played a critical role in the genesis and development of renal cell carcinoma and could be used as a marker of diagnosis and prognosis of renal carcinoma.
作者
程帆
高颖
胡云飞
张孝斌
鲁德银
Cheng Fan;Gao Ying;Hu Yunfei(Department of Urology,First Affiliated Haspital,Hubei Medical University,Wuhan 430060,China)
