摘要
应用GPPCR技术对40例宫颈癌活检组织进行HPV感染的检测,发现有35例为阳性(阳性率为87%),同时利用PCRSSCP(单纯DNA构象多态性)分析技术,对35例HPV阳性的宫颈癌组织进行P53基因5~6外显子的检测,其突变率仅为2-85%(1/35),而在5例HPV阴性的宫颈癌组织中未检测到P53基因突变,结果表明:P53基因的突变在宫颈癌的发生过程中不起主要作用,并且与HPV的感染无相关性。
We applied GP PCR(general primers PCR)to detecting HPV infection in 40 human cervical carcinoma.The postive rate was 87%(35/40).P53 gene 5~6 exon was detected in 35 HPV infection biopsytissues by PCR SSCP analysis.The mutation rate was only 2.85%(1/35).But P53 gene mutation was not detected in 5 human cervical carcinoma without HPV infection.The findings show P53 gene mutation doesn't play an important role in human cervical carcinoma and has no correlation with HPV infection.
作者
邱小萍
伍欣星
谭云
丁晓华
戴天力
赵文先
Qiu Xiaoping;Wu Xinxing;Tan Yun(Virus Research Institute,Basic Medical College,Hubei Medical University,Wuhan 430071China)
基金
省科委“八·五”攻关课题,批准号:922P1701
