摘要
将初步纯化属小鼠ＩｇＧ１亚类的抗Ｇ３单克隆抗体（ＭＧ３１－随机偶联于ＣＮＢｒ－Ｓｅｐｈａｒｏｓｅ４日凝胶，并成功地定向偶联于ｒＰｒｏｔｅｉｎＡ－ＳｅｐｈａｒｏｓｅＦＦ凝胶，两种偶联反应的结合率分别达到９１．７％和８６．６％。ＭＧ３１－ｒＰｒｏｔｅｉｎＡＳｅｐｈａｒｏｓｅＦＦ的抗原结合容量是ＭＧ３１－ＣＮＢｒ－Ｓｅｐｂａｒｏｓｅ４Ｂ的１．８倍，因此，单克隆抗体通过其Ｆｃ区与ｒＰｒｏｔｅｉｎＡ结合，留出正确定向的抗原结合位点，能够提高免疫亲和吸附剂的结合容量。
e
coupled anti - G3 McAb (MG31, IgG1) purified by ammonium sulphate precipitation method to
CNBr - Sepharose 4B gel at random , and to rProtein A - Sepharose FF gel by site - directed
immobilization technique successfully. The binding rate of two coupling reaction were 91.7%
and 86.6% respectively. The antigen binding capacity of MGd31 - rProtein A - Sepharose FF was
1.8 fold that of MG2-CNBr - 8epharose 4B, thus the binding of McAbs via their Fe portions to
rProtein A , leaving the antigen - combining site in thecorrect orientation for the binding of
antigen , could increase the antigen binding capacity of the immunoaffinity chromatography
column
出处
《重庆医科大学学报》
CAS
CSCD
1999年第2期132-134,共3页
Journal of Chongqing Medical University
关键词
免疫亲和色谱
定向偶联方法
单克隆抗体
lmmunoaffinity chromatography
Site - directed coupling method
Monoclonal
anti- body
