摘要
采用给雄性家兔注射黄牛、水牛、山羊ＩｇＧ混合液（１∶１∶１）制备的兔抗黄牛、水牛和山羊ＩｇＧ连接酶标ＳＰＡ，解决了酶标ＳＰＡ与牛羊血吸虫和肝片吸虫免疫复合物相结合的技术关键，并制成了可同时用于牛羊血吸虫病和肝片吸虫病诊断的酶试剂。建立了Ｄｏｔ－ＥＬＩＳＡ同时检测牛羊血吸虫病和肝片吸虫病技术，能测出人工感染１～１５０条虫体７～４２ｄ病畜抗体，阳性检出率达１００％。
A key technique on combining enzyme labelled SPA with immunological complex of Schistosoma and Fasciola hepatica in cattle and sheep was solved by means of enzyme labelled SPA joined to rabbit antiIgG of yellow cattle buffalo and goat. An enzyme agent for simultaneous diagnosis of schistosomiasis and fascioliasis in cattle and sheep was formulated. The developed technique on simultaneous diagnosis of schistosomiasis and fascioliasis in cattle and sheep by DotELISA is able to detected the antibodies from the animals artificially infected with 1150 Schistosoma or F.hepatica 742 days post infection with the positive rate for both Schistosoma and F.hepatica being at 100%.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第3期273-274,共2页
Chinese Journal of Veterinary Science
