摘要
本文研究了磷脂酶D催化合成磷脂酰丝氨酸的工艺,实验结果表明有机相和水相比例、磷脂酰胆碱(PC)浓度、反应温度、水相pH以及反应时间都对磷脂酰丝氨酸的制备有一定的影响。通过正交实验优化利用磷脂酶D催化合成磷脂酰丝氨酸的最佳工艺为:有机相和水相的比例为4/4、PC浓度为75mg/mL、反应温度为40℃、水相pH值为4.0、反应时间为12h,在此工艺条件下磷脂酰丝氨酸得率为:68.9%。
The synthesis conditions of phosphatidylserine were studied using phospholipases D as biocatalyst.Results showed that the reaction time,the pH of water phase,the phosphatidylcholine concentration,the temperature of reaction and the ratio of organic phase to water phase had influence on the reaction.The orthogonal test showed that the best organic solvent-water ratio,phosphatidylcholine concentration,reaction temperature and time were 4/4,75 mg/mL,70 ℃,and 12 h,respectively,under which the yield of phosphatidylserine amount was 68.9%.
出处
《现代食品科技》
EI
CAS
2010年第9期994-996,共3页
Modern Food Science and Technology
关键词
磷脂酶D
磷脂酰丝氨酸
制备
phospholipases D
phosphatidylserine
preparation
