摘要
目的探索一种更为简便安全的下肢动脉缺血性疾病的基因治疗途径。方法建立兔后肢缺血动物模型，将体外构建的重组大肠杆菌质粒ｐＳＶＶＥＧＦ１６５１ｍｇ分５点注射于缺血肌群，３０天后切取标本，测定毛细血管密度和毛细血管数／肌肉数比值。结果经ｐＳＶＶＥＧＦ１６５治疗后，缺血肢体毛细血管密度和毛细血管数／肌肉数比值显著增加，小腿部位比大腿部位增加明显（Ｐ＜００５）。结论肌肉内原位注射ｐＳＶＶＥＧＦ１６５是一种简单有效的治疗基因的转染途径，能显著增加缺血肢体毛细血管形成。
Objective To investigate an easier and safer clinical method of gene therapy for treating arterial ischemic diseases in the lower extreamity. Methods Recominant plasmid pSV VEGF 165 constructed in vitro was directly injected into the ischemic hindlimb muscles in rabbit models. 30 days after gene transfer, the capillary density and the capillary to muscle fiber ratio were determined histochemically in the resected samples. Results The capillary density and the capillary to muscle fiber ratio were significantly higher for pSV VEGF 165 transfected group than for control group,particularly increased in the shank of the hindlimbs. Conclusions Direct intramusclar injection of pSV VEGF 165 was an easier and efficient gene therapy method, and it significantly augmented the capillariy formation in ischemic limbs.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第4期211-213,共3页
Chinese Journal of Surgery
基金
国家自然科学基金
关键词
内皮生长因子
动脉闭塞性疾病
下肢
基因治疗
Endothelial growth factor DNA, recombinant Gene therapy Arterial occlusive diseases
