摘要
目的研究携带白细胞介素１２（ＩＬ１２）基因逆转录病毒载体对体内肝癌生长抑制作用，探索基因治疗肝癌新途径。方法构建携带ＩＬ１２的逆转录病毒载体，转染逆转录病毒包装细胞系ＰＡ３１７，应用该包装细胞对实验性肝癌大鼠进行治疗，观察抗肿瘤作用，免疫功能变化。结果将包装细胞ＰＡ３１７ＩＬ１２肝癌局部或脾内注射后能明显抑制肝癌细胞系ＣＢＲＨ３的生长，接种ＣＢＲＨ３第１、３天注射ＰＡ３１７ＩＬ１２者能长期生存，第５、７天注射者生存期明显延长。而空白对照组及携带空载体的逆转录病毒包装细胞系均可见肿瘤生长（Ｐ＜００１）。结论肝癌局部及脾内注射携带ＩＬ１２基因逆转录病毒载体的包装细胞株均能产生有效抗肝癌作用。
Objective To investigate the inhibitory effects of retrovirus vector containing IL 12 gene on hepatoma growth in vivo and to explore a new approach of gene therapy to hepatocellular carcinoma(HCC). Methods Retrovirus vector containing IL 12 gene was constructed and transfected into packaged cell PA317. Positive PA317 was injected into the rat which suffered from experimental HCC and its anti tumor effects and immunity changes were recorded. Results The packaged cell PA317 containing IL 12 gene could inhibit the proliferation of hepatocellular cell line CBRH3. The rats injected at day 1 or 3 can survive permanently, while those injected at day 5 or 7 can survive longer than those not injected. However, tumor generated in rats injected with blank control or package cells containing retroviral vector without IL 12 gene( P <0 01). Conclusions Package cells transfected with retroviral vector containing IL 12 either injected to the hepatoma tissue locally or given splenically exercises anti hepatoma effects efficiently. The direct intrasplenical injection route is new, safe and effective.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第4期202-204,共3页
Chinese Journal of Surgery
基金
国家自然科学基金
上海市现代生物与新药产业发展基金
