摘要
为了解角质形成细胞作靶细胞进行基因转染、基因表达的可行性，用ｐＳＬＸＣＭＶβｇａｌ转染原代皮肤角质形成细胞，并观察其瞬时表达情况，发现转染后３６小时细胞即可表达β半乳糖苷酶（βｇａｌ），表现为细胞蓝染，４８小时蓝染细胞最多，约占总细胞数的１５％。提示外源基因能够在角质形成细胞中有效地表达。
To investigate the possibility of keratinocytes as a target cell for gene transfection and gene expression, primary keratinocytes were transfected with pSLXCMV βgal and the transient expression of β galactosidase(β gal) was detected The results revealed that β gal was expressed in some cells 36h after transfection, and reached a high level at 48h after transfection with the percentage of positive cells of 1 5% Our results suggested that exogenous genes can effectively express in the keratinocytes
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
1999年第2期71-73,共3页
Journal of Clinical Dermatology
基金
国家自然科学基金
