摘要
为研究内蒙古地区汉族ACE基因第16内含子多态性与糖尿病及合并肾病的相关性,利用PCR技术检测内蒙古地区86例汉族对照组、2型糖尿病(type2 diabetec mellitus,T2DM)不伴有肾病患者(nephropa-thies,DN-)和T2DM合并肾病患者(DN+)的ACE基因第16内含子是否存在插入、缺失(I/D)多态性.结果表明,对照组与DN-组比较,I/D基因型和等位基因频率均没有统计学意义(P>0.05);对照组与DN+组比较,I/D基因型和等位基因频率均有统计学意义;DN+组的ACE基因DD型基因频率和等位基因D显著大于对照组和DN-组,有统计学意义(P<0.05).这表明在内蒙古地区2型糖尿病患者中存在ACE基因第16内含子插入/缺失多态,D等位基因的出现使DN发生的相对危险增加,与II基因型相比,基因型为ID、DD的DM患者并发DN的可能性增加.
The aim of this study was to explore the association between insertion/deletion（I/D） polymorphism of Alu sequence in intron 16 of angiotensin converting enzyme gene and the Patients of T2DM with and without nephropathy.Eighty-six Chinese subjects of Han nationality from Inner Mongolia were classified into control,type2 diabetes mellitus without nephropathy（DN-） and type2 diabetes mellitus with nephropathy（DN＋） groups.Genotypes of polymorphic sites in ACE insertion/deletion（I/D） were determined by PCR technique.The data were analyzed by statistics methods.Allele frequency of I/D was not different between control group and DN-group（P〉0.05）,while there was significantly different between control group and DN＋ group.Distribution of DD and Ⅱ genotype was significantly different between control and DN＋ group.As was ID genotype,while the frequency of D allele and genotype DD was significantly higher in DN＋ group than in control group and DN-group（P〈0.05）.Polymorphic sites in ACE insertion/deletion（I/D） were existed in type2 diabetic patients.Insertion/deletion（I/D） in ACE gene may contribute to genetic susceptibility to DN in T2DM.
出处
《内蒙古师范大学学报（自然科学汉文版）》
CAS
2010年第5期505-509,共5页
Journal of Inner Mongolia Normal University(Natural Science Edition)
基金
内蒙古自然科学基金资助项目(200508010922)
关键词
2型糖尿病
糖尿病肾病
血管紧张素转换酶
I/D多态性
type2 diabetes mellitus
diabetic nephropathies
angiotensin converting enzyme
I/D polymorphism
