摘要
BMPR-IB基因为绵羊产羔数的主效基因,以中国美利奴羊(军垦型)多胎品系为材料,利用PCR-SS-CP、PCR-RFLP和基因测序分析BMPR-IB基因5′非翻译区、编码区和3′非翻译区的单核苷酸多态性(SNPs),研究该基因SNP位点与绵羊高繁殖力的相关性。结果表明,BMPR-IB基因5′非翻译区不存在多态性,编码区存在2个碱基突变(746A→G和1113C→A),3′非翻译区存在1个碱基突变(1354A→G)。最小二乘模型分析表明,A746G突变对绵羊高产羔数的影响达到极显著程度(P<0.001),A1354G突变对绵羊高产羔数的影响差异不显著(P>0.05)。说明A746G位点能够用于高繁殖力中国美利奴羊(军垦型)多胎品系的选择。
BMPR-IB gene（Bone morphogenetic protein type IB） was a major gene controlling litter size in sheep.The SNPs in 5′ UTR,CDS and 3′ UTR of BMPR-IB gene were detected by PCR-SSCP,PCR-RFLP and sequencing in order to find the association between genotypic SNPs and high prolificacy in Chinese Merino（JunKen type） prolific lines.The result showed that no SNPs in 5′UTR,two SNPs（A746G and C1113A） in coding region and one SNP（A1354G） in 3′UTR were detected.Least square means（LSM） analysis showed that there was significant association between the different genotypes of BMPR-IB A746G site and litter size in sheep（P0.001）.There was no association between the different genotypes of BMPR-IB A1354G site and litter size in sheep（P0.05）.These results intensively indicated that BMPR-IB A746G site could be used as a molecular marker of high prolificacy in Chinese Merino（JunKen type）.
出处
《西北农业学报》
CAS
CSCD
北大核心
2010年第9期7-11,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金项目(30860187)
新疆生产建设兵团院士专项(2007JC20)
新疆生产建设兵团农业科技攻关项目(2009GG17)
