摘要
目的:探讨结核性胸液中CD3+ T、CD3+ CD4+ T、CD3+ CD8+ T细胞和CD4+/CD8+比值及细胞因子表达。方法:分离外周血单个核细胞和胸液细胞,流式细胞仪检测CD3+、CD3+ CD4+、CD3+ CD8+和CD4/CD8比值。ELISA法检测胸液上清中IFN-γ、IL-2和TNF-α的水平。流式细胞仪检测CD3+ CD4+和CD3+ CD8+ T细胞Th1细胞因子IL-2、TNF-α、IFN-γ的产生。结果:与外周血相比,胸液细胞CD3+ CD4+ T和CD3+ T细胞比例显著增加。ELISA结果表明,胸液上清中存在大量Th1型细胞因子。流式细胞仪分析结果表明,IL-2、TNF-α和IFN-γ主要由CD3+ CD4+ T细胞产生,而CD3+CD8+T细胞较少或不分泌细胞因子。结论:结核病人胸液中CD3+和CD3+ CD4+ T细胞比例增加,胸液中存在大量Th1细胞因子,且主要来源于CD3+ CD4+ T细胞亚群,可能在抗结核免疫中发挥重要作用。
Objective：To elucidate the phenotypic and functional characteristics of CD3＋ CD4＋ and CD3＋ CD8＋ T in pleural fluid of patients with tuberculous pleurisy.Methods：PBMCs and PFCs were isolated. The frequency of CD3＋,CD3＋ CD4＋,CD3＋ CD8＋ T cells and the ratio of CD4/CD8 in PFCs were analyzed by FACS. The concentration of IFN-γ,IL-2 and TNF-α in the pleural fluid were analyzed by ELISA.Production of IFN-γ,IL-2 and TNF-α by CD3＋ CD4＋ and CD3＋ CD8＋ T cells was detected by FACS following stimulation with BCG.Results：Higher frequencies of CD3＋ CD4＋ T cells and CD4＋ T/CD8＋ T ratio were demonstrated in PFCs when compared with PBMCs from normal donors and TB patients.ELISA analysis demonstrated that significantly high levels of IFN-γ and TNF-α were detected in pleural fluid.Further analysis by FACS indicated that IL-2,TNF-α and IFN-γ were predominantly secreted by CD3＋CD4＋T cells but not by CD3＋ CD8＋ T cells.Conclusion：The frequencies of CD3＋ and CD3＋ CD4＋ T cells are increased in PFCs. Large amounts of Th1 cytokines are detected,which are produced by CD3＋ CD4＋ T cells,and might play important roles against TB infection.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2010年第9期839-841,共3页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(30872300)
