摘要
目的确定与痛觉调节有关的中脑导水管周围灰质（ＰＡＧ）至脊髓（ＳｐＣ）背角的五羟色胺（５－ＨＴ）能神经元通路。方法用辣根过氧化物酶（ＨＲＰ）逆行追踪与免疫细胞化学相结合的方法对大鼠ＰＡＧ至ＳｐＣ背角的５－ＨＴ能投射纤维予以标记。结果在大鼠ＰＡＧ和延髓中缝核（ＮＲ）内观察到ＨＲＰ阳性与５－ＨＴ阳性的双标记神经元，该纤维可投射到颈、胸、腰段ＳｐＣ；双标细胞以同侧为主，对侧较少。结论实验结果表明，从ＰＡＧ－ＮＲ－ＳｐＣ背角形成一条间接通路；另一条为ＰＡＧ直接向ＳＰＣ背角投射而成的直接通路。
Objectives To determine the 5-HT pathway from periaqueductal gray (PAG) to the dorsal horns of spinal cord (SpC) related to the painful regulation.Methods Using a combining method of HRP retrograde tracing with immunocytochemistry for labelling the serotonergic projections from the PAG to the dorsal horns of SpC.Results Both HRP and 5-HT immunoractivity double-labeled neurons were observed in the PAG and nucleus raphe (NR) of rats.The HRP and 5-HT fibers that project to SpC were found in the dorsal horns of the cervical,thoracic and lumbar segments of the SpC.And the double-labeled neurons on ipsilateral side were much more than that on contralateral side.Conclutions The results showed that there might be a connecting way for PAG-NR-SpC indirect pathway which relayed at NR,the other is a direct pathway that PAG neurons project to dorsal horn of SpC.
出处
《宁夏医学杂志》
CAS
1999年第7期387-388,共2页
Ningxia Medical Journal
关键词
中脑
导水管周围灰质
脊髓背角
痛觉调节
5-HT
Mesencpheclin,periaqueductalgray
Spinal cord related
Immunoenzyme techniques
