摘要
目的：探讨脑皮质细胞凋亡在新生猪缺氧缺血性脑病（ＨＩＥ）发生与发展。方法：应用流式细胞术（ＦＣＭ）、ＤＮＡ凝胶电泳对新生猪ＨＩＥ脑皮质细胞凋亡进行定量分析。结果：缺氧缺血后７２小时新生猪左额顶大脑皮质细胞凋亡率较对照组明显增加（ｎ＝１５，１２；ｍａｉｎ±ｓ％＝２６１８±１６３５，３２３±２９３；Ｐ＜００００１）；ＤＮＡ凝胶电泳显示明显“ＤＮＡＬａｄｄｅｒ”。结论：脑皮质细胞凋亡在患ＨＩＥ新生动物的迟发性神经细胞损伤中占有重要地位。
Objective:To determine whether there is apoptosis in cerebral cortical cells of the neonate pigs with hypoxia-ischemia(HI)injury.Methods:We have abserved the DNA Ladderby gel electrophoresis and the apoptosis rate of cerebral cortical cells via flow cytometry.Results:The results showed the apoptosis ratio of cerebral cortical cells in left frontotemporal corticocerebrum 72hr post asphyxia was higher than control group(n=15,12;means%)=26181635,323293;P<00001).Conclusion:It suggest that apoptosis of cerbral cortical cells plays an important role in the delayed cerebral damage of the neonates post HI.
出处
《华西医学》
CAS
1999年第2期142-144,共3页
West China Medical Journal
