摘要
利用高效凝胶色谱(high performance gel permeation chromatography,HPGPC)分析桑叶多糖两个组分MPL1和MPL2的重均分子质量分别为11800D和7630D,利用GC分析MPL1和MPL2是由D-果糖(D-Flu)、L-阿拉伯糖(L-Ara)、L-鼠李糖(L-Rha)、D-木糖(D-Xyl)和D-葡萄糖(D-Glu)5种单糖组成,其物质的量比分别为58:9.9:5.8:5.1:21.2和45:6.74:17.2:7.3:24.1。而通过高碘酸氧化和Smith降解反应的方法得到MPL1和MPL2的主链是由1→3位键合的糖基组成,支链为1→2位键合的糖基组成。红外光谱分析桑叶多糖中存在α构型的C—H吸收峰。
In the present study,we characterized the composition and structural attributes of two polysaccharide fractions （named MPL1 and MPL2） in mulberry leaves obtained after microwave-assisted extraction and Sephadex G50 purification.The HPGPC analysis showed that the weight-average molecular weights of MPL1 and MPL2 were 11800 D and 7630 D,respectively.According to the GC analysis,both polysaccharide fractions were composed of five monosaccharide,namely D-Flu,L-Ara,L-Rha,D-Xyl and D-Glu,and their molar ratio was 58：9.9：5.8：5.1：21.2 for MPL1 and 45：6.74：17.2：7.3：24.1 for MPL2.By using the classic methods of periodate oxidation and Smith Degradation reaction,both the backbones of MPL1 and MPL2 were found to consist of 1 →3 glycosyl bonds and the side chains consisted of 1 →2 glycosyl bonds.C-H absorption peak of α configuration was contained in both polysaccharide fractions according to the IR analysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第17期136-140,共5页
Food Science
