摘要
考察了诱变因素对除虫链霉菌的产量变异效应，从中选择了４种有效的诱变处理方式，并应用于该菌的诱变育种。通过引入循环筛选新方法，仅经４轮诱变筛选程序，得到了３株ａｖｅｒｍｅｃｔｉｎ高产菌，其中ＩＰ８４２突变株在２７℃经５ｄ摇瓶ａｖｅｒｍｅｃｔｉｎＢ１ａ效价可达到４２１ｍｇ／Ｌ，并具有较好的遗传稳定性。
The effects of various mutagenic agents on the
productivity of avermectin by Streptomyces avermilitis were evaluated in this work. Four
different mutagenic breeding methods were developed, which were efficient for positive
mutation of S.avermilitis. After four rounds of cyclic screening, three strains were obtained,
which are the more effective producer of avermectins. The avermectin B1a accumulation
reached 421 mg/L after 5 days fermentation at 27 in shake bottle. The strains were also genetic
stable.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1999年第3期175-177,236,共4页
Chinese Journal of Antibiotics
