摘要
目的探讨吸入一氧化氮（NO）对革兰氏阴性杆菌内毒素（LPS）诱发的急性肺损伤（ALI）肺泡毛细血管膜的影响。方法在机械通气条件下观察吸入NO对损伤肺的氧合功能、肺动态顺应性（Cdyn）、气道阻力（Rrs）及支气管－肺泡灌洗液（BAIF）中蛋白含量（TP）、中性白细胞（PMN）记数及肺湿／干重比（W／D）的影响和形态学检查。结果与对照组比较，吸入NO组治疗6h后的氧合指数（PaO2／FiO2）为37．5±1．8kPa比29．2±3．4kPa、肺内分流率（Qs／Qt）为（18．4±0．99）×10－2比（24．10±1．97）×10-2、肺动态顺应性（Cdyn）为6．53±0．31ml／kPa比5．41±0．41ml／kPa，均较求治疗组有显著改善（P＜0．01）。吸入NO组BALF中TP含量显著降低，为84．0±9．0mg／kg比100．9±7．3mg／kg、PMN记数为（13．83±1．125）×106／L比（19．78±1．289）×106／L，肺湿／干重比（W／D）为3．83±0．13比4．37±0．11。形态学示肺内PMN浸润和水肿减轻。结论吸入NO可显著减轻肺泡毛细血管膜的损伤，对ALI的病程有显著的影响。能为综合治疗提供宝贵的时机。
Objective To examine the effects of inhaled nitric oxide on alveolar-capillary membrane in septic acure lung injury Methods An animal model of acute lung injury induced by endotoxin was established in rabbits under mechanical ventilation .The effects on oxgenation'dynamic compliance (Cdyn). and total protein (TPL)PMN contents in BALF was observed . Results The results showed that inhalation of NO for 6 h significantly improved PaO2/FiO2qS/Qt and Cdyn compared with untreated group PaO2/FiO2(37.5 ± 1 .8)kPa vs (29.2± 3.4)kPa: Qs/Qt (18.4 ± 0.99) × 10-2 vs (24. 10 ± 1 .97) × 10-2 。Cdyn (6.53 ± 0.31 )ml/kPa vs (5.41 ± 0.41 )ml/kPa,redhuced TPL, PMNin BALFand W/D of the lung , TP(84.0 ± 9.0)mg/kg vs (100.9 ± 7.3) mg／kg.PMN(13.83 ± 1. 125) × 106／L vs (19.78 ± 1 .289) × 106／L, andW/D(3.83 ±0.13) vs (4.37 ±0.11)]. Conchusion Inalation of NO can protect alveolar -capillary membrane in septic ALI,is a promising therapy which may provides opportunity for treatment of ALI.
出处
《中国急救医学》
CAS
CSCD
北大核心
1999年第6期326-328,共3页
Chinese Journal of Critical Care Medicine
