摘要
目的：研究重组ＲＡ５３８腺病毒对胃癌细胞的体内外生物学作用。方法：采用ＬａｃＺ基因Ｘ－ｇａｌ染色、ＭＴＴ、克隆形成试验、ＤＮＡ梯度降解试验、原位末端标记、流式细胞仪、裸鼠致瘤性、裸鼠皮下移植瘤模型实验等方法，对ＲＡ５３８重组腺病毒在人胃癌ＳＧＣ７９０１细胞系中的作用进行体内外研究。结果：Ａｄ－ＲＡ５３８对ＳＧＣ７９０１细胞产生明显的生长抑制作用，ＭＴＴ显示生长抑制率为７６．３％。ＤＮＡ梯度降解试验、原位末端标记、流式细胞仪显示Ａｄ－ＲＡ５３８诱导了ＳＧＣ７９０１细胞凋亡。经Ａｄ－ＲＡ５３８处理的ＳＧＣ７９０１细胞裸鼠致瘤性消失。Ａｄ－ＡＳｃ－ｍｙｃ对裸鼠皮下移植瘤模型瘤内注射能有效降低肿瘤的生长速度，生长抑制率为６０．６６％。结论：Ａｄ－ＲＡ５３８对胃癌细胞具有显著的体内外生长抑制及凋亡诱导作用。
Objective: To investigate the
biological effects of recombinant adenovirus RA538 on human gastric cancer cell line in vivo
and in vitro. Methods: AdLacZ Xgal staining, MTT, cloning, DNA ladder, Tunel, FCM,
tumorigenicity in nude mice and model of subcutaneously transplanted tumor in nude mice
were used in this study. Results: AdRA538 strongly inhibited cell growth and induced
apoptosis of SGC7901 cells. The growth rate of the adRA538infected SGC7901 cells was
inhibited for 76.3%. The tumorigenicity in nude mice showed that all the mice failed to form
tumor from 7 to 30 d comparing with AdLacZ and parent SGC7901 cells. Treatment in the nude
mouse model bearing subcutaneous tumor of SGC7901 cells showed that intratumoral
instillation of AdRA538 inhibited the tumor growth. Conclusion: AdRA538 can inhibit growth
and induce apoptosis of gastric cancer cell in vivo and vitro.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第5期309-313,共5页
Journal of Third Military Medical University
基金
国家863计划资助
关键词
腺病毒
基因治疗
维甲酸
胃肿瘤
RA538
adenovirus
gene
therapy
gastric carcinoma
retinoic acid
nude mouse
