摘要
目的：探讨改良的凝胶阻滞法用于核蛋白-DNA结合的检测。方法：培养人真皮成纤维细胞，用维甲酸刺激不同时段，抽提其核蛋白，与32P-标记维甲酸反应元件（RARE）及维甲类X受体α、β（RXRα、β）抗体结合，进行凝胶阻滞分析。结果：细胞于不同状态可出现迁移率不同的条带，加入RXRβ抗体可产生新的上移条带。结论：改良后的凝胶阻滞法较简单灵敏，可用于核蛋白-DNA结合的检测。
To improve a gel retardation Assay for the study of binding in the nuclear protein and DNA. Method: Cultured fibroblasts were stimulated with Retinoic acid in different time courses, nuclear protein was iwhated and binded with 32P labeled retinoic acid responsive element and antibody to Retinoid-X Receptors α, β (RXRα, β), and then gel retardation assays were performed. Result: There were different bands which resulted from different mobility in electrophoresis when the cells were in different conditions. A new supershift band appeared after incubating the antibody to RXR β with nuclear protein. Conclusion: The improved gel retar-dation assays suitable for the study in dinding of nuclear protein and DNA are simple and sensitive.
出处
《广西医科大学学报》
CAS
1999年第1期25-27,共3页
Journal of Guangxi Medical University
关键词
凝胶阻滞分析
核蛋白
维甲类X受体
DNA
gel retardation assays
nuclear protein
retinoid X Receptors (RXR)
