摘要
采用溶胶-凝胶法制备了氧化硅-氧化锆复合氧化物(SiO2-ZrO2),并对其物理与化学性质进行了详细地表征。以α-酪蛋白的酶解产物为探针,比较了氧化硅-氧化锆复合氧化物与氧化锆对磷酸化肽段的富集能力。结果表明:在pH<2时富集,SiO2-ZrO2对磷酸化肽的选择性要优于ZrO2;使用SiO2-ZrO2材料时,多磷酸化肽段的回收率更高。结合MALDI-TOFMS检测技术,SiO2-ZrO2成功地应用于脱脂牛奶酶解产物中磷酸化肽的分离和富集。
Silica-zirconia composite（SiO2-ZrO2） was synthesized by sol-gel method and its physical-chemical properties were investigated with several techniques.The enrichment capacity of silica-zirconia composite and zirconia was compared using tryptic digest of α-casein as probe.The results showed that the selectivity of SiO2-ZrO2 was better than that of ZrO2 when pH value of loading solution was 2.The enrichment capacity of SiO2-ZrO2 for multiple phosphorylated peptides was better than that of ZrO2.In combination with MALDI-TOF based peptide mass finger printing technique,SiO2-ZrO2 was used to enrich the phosphopeptides from nonfat milk successfully.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第9期1231-1237,共7页
Chinese Journal of Analytical Chemistry
基金
国家科技部973计划(No2007CB914200)资助项目
关键词
氧化硅-氧化锆复合氧化物
磷酸化肽段
富集
基质辅助激光解吸飞行时间质谱
Silica-zirconia composite
Phosphopeptide
Enrichment
Matrix assisted laser disorption ionization-time of flight-mass spectrometry
