摘要
１ ０２４羽３００日龄罗曼蛋鸡随机分４组，分别饲喂二氢吡啶（Ｄｉｌｕｄｉｎ）添加量０，１００，１５０，２００ ｍｇ／ｋｇ的４种饲粮，于饲喂后第３０日和第８０日采血和屠宰，研究其对蛋鸡内分泌功能的影响。结果显 示：Ｄｉｌｕｄｉｎ均显著地提高了腺垂体和血清中ＦＳＨ，ＬＨ含量，其中垂体ＦＳＨ和ＬＨ含量随饲喂剂量提高 而增加，血清ＦＳＨ和ＬＨ含量以１５０ ｍｇ／ｋｇ组效果最佳；Ｄｉｌｕｄｉｎ提高了血清Ｔ３含量，Ｔ４差异不显著， ３０日Ｔ３随饲粮中Ｄｉｌｕｄｉｎ递增而增加，８０日１５０，２００ ｍｇ／ｋｇ组与对照组差异显著；饲粮中添加Ｄｉｌｕｄｉｎ 具有改进血清雌二醇（Ｅ２）水平，其中８０ 日 １５０，２００ ｍｇ／ｋｇ组与对照组差异显著；１５０ ｍｇ／ｋｇ Ｄｉｌｕｄｉｎ显 著提高血清中孕酮（Ｐ４）含量；饲粮中添Ｄｉｌｕｄｉｎ显著提高下丘脑和腺垂体中ｃＡＭＰ含量。
The experiment was conducted to study the effects of Diludin on endocrnology of laying hen. 1024 ROM hens were divided into 4 groups, each of which included four replicates of 64 hens each ,The control group received the basal diet ,and the other 3 received the same basal diet supplemented with 100 , 150 and 200 mg/kg Diludin respectively. After 30 d and 80 d 16 hens of each treatment were picked out to be slaughtered , Samples of blood,hypothalamus and pituitary were collected for hormones analysis. Addi- tion of Diludin to diet significantly increased the concentrations of follicle-stimulating hormone (FSH) and luternizing hormone (LH) in anterior pituitary and serum. The concentrations of FSH and LH in anterior pituitary were increased with the increasing of Diludin added , and the level of FSH and LH in serum were highest in 150 mg/kg treatment. The level of triiodothyronine (T3) in serum were significantly increased with the increasing level of Diludin in 30 d , and in 80 d the level of T3 in serum were significantly im- proved in 150 and 200 mg/kg treatments. No significant difference was observed in thyroxine of serum in each treatments. Adding Diludin to diet improved the level of estradiol (E2) in serum, and it was signifi- cantly increased than control in 150 and 200 mg/kg treatment in 80 d . Adding with 150 mg/kg Diludin significantly increased serum progesterone (P4) concentrations. Addinq Diludin to diet significantly in- creased concentrations of cAMP in hypothalamus and anterior pituitary.
基金
浙江省科委基金!981102028
