摘要
[目的]油松苗期发生的猝倒病是油松育苗工作的极大障碍,对该病害病原菌的正确鉴定是研究其致病机理、开展防治工作等的基础。[方法]对1株引起油松苗猝倒病的病原真菌通过形态学、培养性状、致病性及rDNA ITS序列测定来鉴定该病原菌。[结果]在PDA培养基上,该病原菌幼嫩菌丝无色,但在生长过程中逐渐变为褐色,部分菌丝膨大成念珠状;菌丝细胞多核;菌丝平均直径6~10μm,分支发生点附近缢缩并形成一隔膜,分支与再分支呈直角;不产生无性孢子,也不产生菌核;能引起油松苗猝倒病;其rDNAITS序列与瓜亡革菌（Thanatephorus cucumeris）的2个菌株的相似性最大,均为97%。[结论]该病原菌被鉴定为立枯丝核菌（Rhizoctonia solaniKhn）。该研究可为后续试验的进行奠定基础。
[Objective] Pinus tabulaeformis damping-off has brought serious losses to P.tabulaeformis seedling and reforestation,a correct identification of the pathogens of damping-off is a basis for studying the pathogenic mechanism and controlling the disease.[Methods]In this study,a pathogenic fungus of P.tabulaeformis seedling damping-off was identified by investigating the pathogenic fungus morphology,cultural characteristics,pathogenicity and rDNA ITS sequence analysis.[Result] Young hyphae of the fungus was colourless,it produced white to brown mycelium when grown on PDA medium.Its hyphal cells were multinucleate,the hyphae were 6-10 μm wide and tended to branch at right angles,a septum was near each hyphal branch and a slight constriction at the branch;it did not produce spores and sclerotia;it could cause P.tabulaeformis seedling damping-off;its rDNA ITS sequences had highest similarity with two strains of Thanatephorus cucumeris,both similarity were 97%.[Conclusion] The pathogen was identified as Rhizoctonia solani Kühn.This study laid a foundation for the future researches.
出处
《安徽农业科学》
CAS
北大核心
2010年第20期10768-10769,10782,共3页
Journal of Anhui Agricultural Sciences
基金
青岛农业大学高层次人才启动基金项目(630901)
关键词
油松
形态学
致病性
RDNA
ITS序列
立枯丝核菌
Pinus tabulaeformis
Morphology
Pathogenicity
rDNA ITS sequences
Rhizoctonia solani Kühn
