摘要
[目的]研究仔猪大肠埃希氏菌病、C型产气荚膜梭菌病二联灭活疫苗的质量及产品的安全性。[方法]对大肠埃希氏菌和C型产气荚膜梭菌浓缩菌液,用不同浓度甲醛溶液经不同灭活时间、灭活温度进行了灭活和脱毒试验。[结果]大肠埃希氏菌培养物在37～38℃条件下用0.4%的甲醛溶液灭活48h,可以使细菌全部灭活;C型产气荚膜梭菌培养物在37～38℃,采用0.5%～0.8%的甲醛溶液进行灭活和脱毒7～14d,或者采用2次灭活和脱毒方法,7d可以达到完全灭活和脱毒。[结论]在37～38℃条件下,用0.4%的甲醛溶液对大肠埃希氏菌培养物灭活和采用0.5%～0.8%的甲醛溶液对C型产气荚膜梭菌进行灭活和脱毒,可以达到理想效果。
[Objective] In order to assure the quality and security of Escherichia coli and C-type clostridium wechii combined vaccine.[Method] The different inactive temperature and time were tested on Escherichia coli and Clostridium wechii in different concentration formaldehyde.[Result] Escherichia coli was killed in 0.4% formaldehyde at 37-38 ℃ for 48 h.Clostridium wechii was inactived and losing virulence in 0.5%-0.8% formaldehyde at 37-38 ℃ for 7-14 d.[Conclusion] It can get better effect to Escherichia coli in 0.4% formaldehyde at 37-38 ℃ for 48 h and 0.5%-0.8% formaldehyde at 37-38 ℃ for 7-14 d to C-type Clostridium wechii.
出处
《安徽农业科学》
CAS
北大核心
2010年第20期10702-10703,10728,共3页
Journal of Anhui Agricultural Sciences
基金
湖北省科技厅引导项目(2006AA201C25)
湖北省农业科技创新中心资助项目(2007-620-001-03)资助
关键词
大肠埃希氏菌
C型产气荚膜梭菌
灭活
脱毒
Escherichia coli
C-type Clostridium wechii
Inactivation
Detoxification
