摘要
[目的]获得本生烟草转胰高血糖素样肽-1基因植株。[方法]以本生烟草无菌苗叶片为外植体材料,建立本生烟草再生系统,通过农杆菌叶盘法浸染进行遗传转化,获得本生烟草转胰高血糖素样肽-1基因植株。[结果]通过多重比对和重复,确定本生烟草叶片最适分化培养基为MS+1.5mg/L6-BA+0.1mg/LNAA,生根培养基为MS+0.1mg/LIBA;经由卡那霉素浓度梯度试验,确定选择压力为5mg/L卡那霉素;对经卡那霉素筛选获得的抗性芽进行GUS组织化学检测,获得阳性信号。[结论]初步证实外源基因胰高血糖素样肽-1已整合到本生烟草基因组中。
[Objective]The research aimed to obtain the transfer of glucagon likepeptide-1 gene Nicotiana benthamiana plants.[Method] With sterile tobacco leaf explants as the experimental material,the regeneration system of tobacco was established and transgenic Nicotiana benthamiana was obtained through Agrobacterium infection.[Result]By multiple comparison and repeated experiments,the optimal Nicotiana benthamiana differentiation medium of MS＋1.5 mg/L 6-BA ＋0.1 mg/L NAA was determined,so did the rooting medium MS ＋0.1 mg/L IBA.By kanamycin selection pressure gradient experiments,the selection concentration was identified as 5 mg/L kanamycin,later disseminated by Agrobacterium leaf disc method for genetic transformation.GUS acquired resistance to staining the buds and the positive signal was obtained.[Conclusion]Gene GLP-1 was integrated into the genome of tobacco Nicotiana benthamiana.
出处
《安徽农业科学》
CAS
北大核心
2010年第20期10530-10531,10607,共3页
Journal of Anhui Agricultural Sciences
基金
北京林业大学理科基地基金
关键词
本生烟草
组培
转基因
GUS检测
Nicotiana benthamiana
Tissue culture
Transgenic
GUS detection
