摘要
目的观察庆大霉素慢性耳中毒前后蒙古沙鼠内侧橄榄耳蜗（ｍｅｄｉａｌｏｌｉｖｏｃｏｃｈｌｅａｒ，ＭＯＣ）传出神经的形态改变及其与毛细胞损害的关系，以探讨ＭＯＣ传出神经在氨基糖甙类抗生素慢性耳中毒中的重要性。方法采用改良的乙酰胆碱酯酶组化染色和甲苯胺蓝苏木素染色法，全耳蜗铺片观察健康对照组和庆大霉素组耳蜗ＭＯＣ传出神经和外毛细胞（ｏｕｔｅｒｈａｉｒｃｅｌ，ＯＨＣ）的分布特征，并测量耳蜗ＭＯＣ传出神经纤维、末梢及ＯＨＣ的数量。结果停药后３周出现耳蜗ＭＯＣ纤维和末梢损害，损害程度随观察时间延长而加重，１１周最明显，且损害部位主要在耳蜗底回，与毛细胞损害的特征一致。结论耳蜗ＭＯＣ传出神经可能在庆大霉素慢性耳中毒时ＯＨＣ的损害中起重要作用。
Objective To determine the possible involvement of the medial olivocochlear
efferents (MOC) in chronic aminoglycoside ototoxicity, the morphological changes of the MOC
efferents in the cochlea of Mongolian gerbils and their correlations with the impairment of the
outer hair cells (OHCs) were observed. Methods The animals were given gentamicin in a dose
of 150 mg/kg a day for 10 days. The distributions and densities of the MOC efferents were
examined using the modified histochemical staining for acetylcholinesterase (AChE) and the
numbers of OHCs were numerated with toluidine blue and Ehrlich haematoxylin staining on the
surface preparation. Results Significant damage to the MOC fibers and terminals as well as
OHCs was noted in the basal turn of the cochlea, especially in those animals examined in 7th
and 11th week after the gentamicin administration. The site of the greatest impairment of OHCs
was consistent with that of the MOC efferents. Conclusion The results suggested that damage
to the MOC efferents might play an important role in gentamicininduced impairment of OHCs.
出处
《中华耳鼻咽喉科杂志》
CSCD
1999年第3期157-159,共3页
Chinese Journal of Otorhinolaryngology
基金
国家自然科学基金
关键词
庆大霉素类
中毒
听力障碍
传出神经元
氨基糖苷
Gentamicins Poisoning Hearing disorders Neurons,efferent Antibiotics,
aminoglycoside
