摘要
目的探讨甘油三酯和极低密度脂蛋白（ＶＬＤＬ）及其刺激的Ｋｕｐｆｅｒ细胞对大鼠肝星状细胞（ＨＳＣ）增殖的影响。方法用链霉蛋白酶和胶原酶原位灌流，Ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠ＨＳＣ和Ｋｕｐｆｅｒ细胞，制备甘油三酯和ＶＬＤＬ刺激的Ｋｕｐｆｅｒ细胞培养的上清液（ＫＣＣＭ），并以ＭＴＴ法观察甘油三酯和ＶＬＤＬ及ＫＣＣＭ对ＨＳＣ增殖效应。结果甘油三酯和ＶＬＤＬ浓度分别在１２５ｍｇ／Ｌ和２５～１００ｍｇ／Ｌ时对ＨＳＣ增殖有促进作用（Ｐ＜００５或Ｐ＜００１），与正常和ＫＣＣＭ组相比，ＫＣＣＭ＋ＶＬＤＬ组和ＫＣＣＭ＋甘油三酯组对ＨＳＣ增殖有促进作用（Ｐ＜００１），ＫＣＣＭ＋ＶＬＤＬ组和ＫＣＣＭ＋甘油三酯组之间差异无显著性（Ｐ＞００５）；ＫＣＣＭ组高于正常组，但统计学无差异（Ｐ＞００５）。结论甘油三酯和ＶＬＤＬ及其刺激的ＫＣＣＭ可促进ＨＳＣ增殖，其与脂肪肝、肝纤维化的发生可能有关。
Objective To study the effects of Kupffer cellconditioned medium (KCCM)
derived from triglyceride and very lowdensity lipoprotein (VLDL) treatment on proliferation of rat
hepatic stellate cells (HSC). Methods HSC and Kupffer cells were isolated from liver of Wistar
rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with
Nycodenz and then cultured. KCCM was prepared and MTT colorimetric assay was used to
detect HSC proliferation. Results HSC and Kupffer cells were isolated successfully with high
purity. 12.5 mg/L of triglyceride and 25100 mg/L of VLDL promoted HSC proliferation (P<0.05 or
0.01); KCCM+triglyceride and KCCM+VLDL might promote HSC proliferation as compared with a
control grouAuthor p (P<0.01). There was no significant difference between KCCM+triglyceride
and KCCM+VLDL (P>0.05). T5HZConclusion The technique for isolation of HSC and Kupffer cells
described here is simple and reliable. Triglyceride, VLDL and KCCM stimulated by them may
promote HSC proliferation and be associated with fatty liver and hepatic fibrogenesis.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1999年第5期313-315,共3页
Chinese Journal of Internal Medicine
基金
国家自然科学基金
