摘要
Objective： To analyze the changes in peripheral natural killer T-cells （NKT） and gammadelta T-cells （γδT-cell） in patients with minimal residual leukemia （MRL） before and after being treated with Yiqi Bushen Granule （益气补肾颗粒, YBG） in order to determine their significance in prognosis of the disease. Methods： Before and after treatment, the changes in 36 patients （16 males and 20 females） receiving long-term （more than 3 months） YBG therapy were analyzed using multi-parameter flow cytometry, with 34 healthy persons （19 males and 15 females） acting as controls. Results： The absolute value and percentage of NKT cells and γδT-cells were all significantly raised after treatment, for NKT cells, 0.52%±0.39% to 0.83%±0.66% and 7.25±7.77 cell/μL to 12.86±11.99 cell/μL, for γδT-cells, 6.08%±3.03% to 7.24%±2.78% and 83.97±48.09 cell/μL to 110.53±54.12 cell/μL, respectively （P0.05 or P0.01）. Conclusion： YBG could regulate the immune function and elevate the amount of NKT cells and γδT-cells, thus to kill or suppress the residual leukemic cell in the body, which might be one of the mechanisms of YBG in prolonging the disease-free survival in MRL patients.
Objective： To analyze the changes in peripheral natural killer T-cells （NKT） and gammadelta T-cells （γδT-cell） in patients with minimal residual leukemia （MRL） before and after being treated with Yiqi Bushen Granule （益气补肾颗粒, YBG） in order to determine their significance in prognosis of the disease. Methods： Before and after treatment, the changes in 36 patients （16 males and 20 females） receiving long-term （more than 3 months） YBG therapy were analyzed using multi-parameter flow cytometry, with 34 healthy persons （19 males and 15 females） acting as controls. Results： The absolute value and percentage of NKT cells and γδT-cells were all significantly raised after treatment, for NKT cells, 0.52%±0.39% to 0.83%±0.66% and 7.25±7.77 cell/μL to 12.86±11.99 cell/μL, for γδT-cells, 6.08%±3.03% to 7.24%±2.78% and 83.97±48.09 cell/μL to 110.53±54.12 cell/μL, respectively （P0.05 or P0.01）. Conclusion： YBG could regulate the immune function and elevate the amount of NKT cells and γδT-cells, thus to kill or suppress the residual leukemic cell in the body, which might be one of the mechanisms of YBG in prolonging the disease-free survival in MRL patients.
基金
Supported by the National Natural Science Foundation of China (No. 30472284)
the Capital Scientific Research Development Fund for Key Medical Program (TCM, No. I-1)
