摘要
目的:建立白花丹参药材的HPLC指纹图谱。方法:采用高效液相色谱法,色谱柱:Diamonsil C18(4.6mm×250mm,5μm),流动相:甲醇-0.1%三氟乙酸水溶液进行梯度洗脱,检测波长270nm,流速1.0mL/min。结果:在选定的色谱条件下,通过中药色谱指纹图谱相似度评价系统(2004A)确定14个色谱峰构成白花丹参药材指纹图谱的特征峰。结论:该分析方法的稳定性、精密度、重现性较好。
】 AIM： To develop an HPLC fingerprint method for the quality control of Salvia Miltiorrhiza f. Alba. METHODS： An HPLC analysis was carried out on a Diamonsil C18 column（4. 6 mm × 250 mm,5 μm） with themobile phase consisting Methanol-0. 1% trifluoroacetic acid water in gradient mode. The detection wavelength was set at 270 nm. The flow rate was 1. 0 mL·min-1 and the analytic time was 80 min. The ten batches of samples were determined,for establishing the standard HPLC fingerprint of Salvia miltiorrhiza f. Alba. RESULTS： Under the above chromatographic conditions the characteristic peak of fingerprint of Salvia miltiorrhiza f. Alba was composed of 14 chromatographic peaks through similarity analysis. CONCLUSION： The method has good reproduc-ibility,precision and stability.
出处
《中成药》
CAS
CSCD
北大核心
2010年第9期1464-1467,共4页
Chinese Traditional Patent Medicine
基金
山东省教育厅科技项目(J04E07)
泰安市科技局课题(KY200530)
关键词
白花丹参
高效液相色谱法
指纹图谱
Salvia miltiorrhiza f. Alba
HPLC
chromatogram fingerprint
