摘要
目的:运用细胞学及实时荧光定量RT-PCR方法对胃癌术中腹腔冲洗液进行检测,以探讨对预测胃癌腹膜微转移的意义。方法:胃癌50例和胃良性病变10例,收集患者术中腹腔冲洗液,用实时荧光定量RT-PCR方法测定腹腔冲洗液中游离细胞的CK20mRNA、COX-2 mRNA表达,同时作冲洗液细胞学检测(PLC)。结果:50例胃癌患者腹腔冲洗液中CK20mRNA阳性表达为62.0%(31/50);COX-2 mRNA阳性表达率60.0%(30/50),CK20mRNA、COX-2 mRNA联合检测阳性率为68.0%(34/50),皆高于腹腔冲洗液细胞学28.2%(11/50),CK20mRNA、COX-2 mRNA的阳性率与肿瘤分期、浸润深度、淋巴结转移、浆膜侵犯程度呈正相关。结论:CK20mRNA、COX-2 mRNA实时荧光定量RT-PCR方法检测腹腔微量游离癌细胞较PLC有更高的灵敏度,是一种检测胃癌腹膜微转移的有效方法。
Objective：To detect the free cancer cells in the peritoneal washes and to explore their signifcances in the prediction of peritoneal metastasis of gastric cancer. Methods： The peritoneal washes were collected from 50 patients with gastric cancer and 5 with benign gastric disease. Peritoneal lavage cytology was performed for the washes, and CK20mRNA,COX-2 mRNA of free cancer cells was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction（fqRT-PCR）. Results： CK20mRNA,COX-2 mRNA positive rates of free cancer cells in peritoneal washes were 62.0%（31/50） and 60.0%（30/30）, respectively, which were both higher than that of cytological examination 28.2% （11/50）. The TNM staging, depth of invasion, lymph node metastasis, serosal involvement were correlated with the positive rate of CK20mRNA,COX-2 mRNA. Conclusion： CK20mRNA,COX-2mRNA detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction is more sensitive for peritoneal micrometastasis of gastric cancer.
出处
《现代肿瘤医学》
CAS
2010年第9期1785-1788,共4页
Journal of Modern Oncology
基金
全军医学科学技术研究"十一五"计划课题项目(编号:06MA082)
关键词
胃癌
微转移
实时荧光定量RT-PCR
肿瘤标志物
PLC
gastric carcinoma
micrometastasis
real-time fluorescent PCR
tumor marker peritoneal lavage cytology
