摘要
目的:观察后处理对肠缺血-再灌注大鼠肺组织凋亡相关基因表达的影响。方法:24只SD大鼠随机分为3组(n=8):假手术(sham)组、缺血-再灌注(I/R)组、缺血后处理(IPOST)组;应用光学显微镜观察各组大鼠肺组织病理学变化,测定肺湿/干比值(W/D)。应用RT-PCR和Western blotting方法分别检测各组大鼠肺组织Bcl-2、Bax和caspase-3mRNA及其蛋白表达的变化。结果:与T/R组相比,IPOST组大鼠肺组织病理学改变明显减轻,肺W/D显著下降(P<0.05),Bcl-2mRNA和蛋白表达水平明显升高(P<0.05),而Bax和caspase-3mRNA与蛋白表达量则明显降低(P<0.05)。结论:缺血后处理可能通过上调Bcl-2mRNA和蛋白表达,下调BaxmRNA和蛋白表达,减轻大鼠肠缺血-再灌注所致的急性肺损伤。
AIM： To investigate the effect of postconditioning on apoptosis-related gene expression of lung tissues in rat intestinal ischemia-reperfusion（ I/R） . METHODS： Male Sprague-Dawley rats were divided into 3 groups which were sham operation group （ sham） ,I/R group and ischemic postconditioning （ IPOST） group. The experimental animals were sacrificed after reperfusion for 6 h. The tissue sections were stained with hematoxylin and eosin and examined under light microscope to observe lung histopathological changes. Lung W/D ratios were measured. Lung tissue samples were taken to detect Bcl-2,Bax and caspase-3 mRNA and protein expression using RT-PCR and Western blotting analysis,respectively. RESULTS： Compared with I/R group,the morphological changes of lung were alleviated and the lung W/D ratios significantly decreased in IPOST group （ P 0. 05） . The mRNA and protein expression of Bcl-2 in IPOST group was obviously higher than that in I/R group （ P 0. 05） ,while the mRNA and protein expression of Bax and caspase-3 in IPOST group was obviously higher than that in I/R group （ P 0. 05） . CONCLUSION： Ischemic postconditioning attenuated intestinal ischemia-reperfusion-induced acute lung injury partly by increasing Bcl-2 mRNA and protein expression,reducing Bax mRNA and protein expression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第9期1786-1789,共4页
Chinese Journal of Pathophysiology
基金
陕西省科技计划资助项目(NoSJ08C205)
西安医学院校级科研基金资助项目(No2007FZ01)
西安医学院博士科研启动基金资助项目(NoSJ08C205)
关键词
缺血-再灌注
缺血后处理
急性肺损伤
细胞凋亡
Ischemia-reperfusion
Ischemic postconditioning
Acute lung injury
Apoptosis
