摘要
目的 探讨肿瘤坏死因子-α（TNF-α）C863A、T1031C基因多态与缺血性脑卒中（IS）的关系.方法 共收集150例IS患者和110例对照者,应用聚合酶链反应一限制性片段长度多态性（PCR-RFLP）方法检测TNF-α基因C863A、T1031C多态性.x2检验分析比较基因型和等位基因频率的差异,多元Logistic回归分析方法分析各种危险因素对IS的影响.结果 IS组和对照组在-863位点CA＋AA基因型频率分别为0.387和0.255,比较差异有统计学意义（χ2=5.004,P=0.025）,IS组A等位基因频率亦高于对照组,比较差异有统计学意义（χ2=5.176,P=0.023）.按性别分组后,发现男性IS组CA＋AA基因型频率和A等位基因频率均明显高于对照组,比较差异有统计学意义（χ2=7.968,p=0.005;χ2=7.557,P=0.006）.-1031位点多态TC＋CC基因型和C等位基因在IS组和对照组差异无统计学意义（χ2=1.463,P=0.226;χ2=2.849,P=0.091）.多元Logistic回归分析显示TNF-α C863A多态是IS的独立危险因素（P=0.022,OR=1.846,95%CI：1.075～3.169）.结论 TNF-α C863A多态与老年IS有相关性,可能是IS危险因素之一,T1031C基因多态与IS无相关.
Objective To explore the relationship between the tumor necrosis factor-α（TNF-α） T1031C and C863A gene polymorphisms and senile ischemic stroke （IS） in elderly patients.Methods One hundred and fifty patients with IS and 110 age and gender-matched controls were recruited in this study. The T1031C and C863A gene polymorphisms were detected by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） assay in these subjects. Data were coded and analyzed in SPSS Windows （version 11.5）： the differences of genotype and allele frequencies were analyzed and compared byx2 test; multiple logistic regression analysis was employed to analyze the effect of various risk factors on IS. Results The frequencies of CA＋AA genotypes （0.387） and A allele （0.203） at the position of-863 in the IS group were significantly higher than those in the control group （[0.255 and 0.127]; [χ2=5.004, P=0.025] and [χ2=5.176, P=0.023]）. Further analysis indicated that the frequencies of genotypes and alleles were statistically different between male IS subgroup （0.430 and 0.227） and controls （[0.212 and 0.106]; [x=7.968, P=0.005] and [χ2=7.557, P=0.006]）. No differences of TC＋CC genotypes and C alleles at the position of-1031 were observed between IS group and controls （[χ2=1.463, P=）.226] and [χ2=2.849, P=0.091]）. Multiple logistic regression analysis revealed that hypertension, diabetes mellitus, hyperlipidemia and the A allele of C863A gene polymorphism were independent risk factors for IS in elderly patients （P=0.022, OR=1.846, 95%CI： 1.075～3.169）.Conclusion The TNF-α C863A gene polymorphism may be an independent risk factor for senile IS.The T1031C gene is unlikely to contribute to the IS.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2010年第9期893-896,共4页
Chinese Journal of Neuromedicine
