摘要
用核内蛋白质与经0.4mol/L NaCL抽提后的细胞核重组,以DNA酶I消化法为测定活性基因的指标,观察猪胸腺HMG_(17)非组蛋白对细胞核中活性基因的影响。结果显示:猪胸腺HMG_(17)使细胞核对DNA酶I作用的敏感性增加,因此认为HMG17为使细胞核胞核中基因呈较大活性的重要因素。
Nonhiston protein HMG17 is purified from pig thymus. Elution of the pig thymus nuclei with 0.4mol/L NaCl leads to slightly lowering in DNase I sensitity of actively transcribedgene. Reconstitution of the depleted nuclei with 0.4mol/L Nacl eluate, total HMG or HMG17 results in successful reconstitution of the DNase I sensitivity of actively transcribed gene. Thus, HmG17 protein is an important factor responsible for DNase I sensitivity.
关键词
HMG非组蛋白
转录活性基因
Nonhiston protein HMG, DNase I digestion, Activily transcribed gene, Ultraviolet absorbence.
