摘要
目的:观察母体铅暴露对仔鼠学习记忆及海马组织内糖原合成酶激酶-3β(GSK-3β)mRNA表达的影响。方法:雌性小鼠40只,自妊娠第1天开始经饮水染铅,高、中、低剂量铅暴露组分别饮用0.3、1.0和3.0g/L的醋酸铅水,对照组饮蒸馏水,至仔鼠出生后第21天断乳为止。抽取各组仔鼠10只,在出生后第7、14、21天分别测其血液和海马组织中铅水平;在出生后第21天时,进行Y型迷宫实验,并采用RT-PCR方法测定海马组织中GSK-3βmRNA的相对表达量。结果:4组仔鼠第7、14、21天血铅和海马组织铅水平随时间和染毒剂量的增加而变化(血清:F组间=315.204,F时间=16.427;F交互=53.125,P均<0.05。海马:F组间=256.205;F时间=7.312;F交互=10.213,P均<0.05);与对照组相比,各剂量铅暴露组血液和海马组织铅水平显著升高(P<0.05)。第21天4组仔鼠的学习记忆能力(T1和T2)差异有统计学意义(F组间=43.312,F时间=72.345,F交互=83.532,P均<0.05),记忆保持率(T3)差异亦有统计学意义(F=18.264,P=0.013);低、中、高剂量铅暴露组T1、T2高于对照组,T3低于对照组(P均<0.05)。4组仔鼠海马组织中GSK-3βmRNA的表达量比较,差异有统计学意义(F=37.263,P<0.001),低、中、高剂量铅暴露组均较对照组明显升高(P<0.05)。结论:母体铅暴露导致铅在仔鼠体内蓄积,蓄积的铅通过诱导海马内GSK-3βmRNA的高表达,造成神经系统功能的损害,从而引起仔鼠学习记忆能力下降。
Aim：To observe the effect of maternal lead exposure on learning and memory and the influence of glycogen synthase kinase-3β（GSK-3β） mRNA expressed in hippocampus of mouse pups.Methods：Pregnant mice were exposed to zero,low-level（0.3 g/L）,moderate-level（1.0 g/L） and high-level （3.0 g/L）lead acetate in demonized drinking water from the first day of gestation to postnatal day 21.The lead concentration in blood and hippocampus at the 7th,14th and 21st day of neonatal mice pups were detected.The ability of learning and memory and the expression of GSK-3β mRNA in hippocampus at the 21st day were measured by Y-type Maze and RT-PCR.Results：The lead concentrations in blood and hippocampus were different among the four groups at the 7th,14th and 21th day （blood lead：Fgroup=315.204,Ftime=16.427,Fbetween=53.125,P0.05;hippocompus：Fgroup=256.205;Ftime=7.312,Fbetween=10.213,P0.05）.They were higher in lead exposure groups than those in the control gorup（P0.05）.The ability of learning and memory （T1 and T2） were different in four groups（Fgroup=43.312,Ftime=72.345,Fbetween=83.532,P0.05）.The memory retaining rate （T3） of the 4 groups was different （F=18.264,P=0.013）,and T3 in the three lead exposed groups significantly decreased （P0.05） compared with the control group.The expression of GSK-3β mRNA in hippocampus were different among the 4 groups（F=37.263,P0.001）.They were obviously higher in lead exposure groups than that in the control group （P0.05）.Conclusion：Maternal lead exposure make the lead accumulated in the mouse pup body,decreasing the function of study and memory.The nerve poison leaded by lead may damage the nerve system function by inducing the high expression of GSK-3β mRNA.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第5期815-818,共4页
Journal of Zhengzhou University(Medical Sciences)
关键词
铅
海马
糖原合成酶激酶-3Β
学习记忆
lead
hippocampus
glycogen synthase kinase-3β
learning and memory
