摘要
主要研究矿质元素对北虫草继代培养中形成的不同类型菌落子实体产量及菌丝生长速度的影响,旨在探讨矿质元素与子实体产量以及菌丝生长速度的关系。采用正交试验,将北虫草优良菌株L20在不同矿质元素培养基上进行12次继代培养。结果表明菌株L20在不同培养基中继代培养后形成7种菌落类型,有子实体形成能力的菌落为Ⅰ、Ⅱ、Ⅲ、Ⅴ型,其中Ⅰ型菌落产量最高、Ⅲ型中等、Ⅱ型最低,Ⅴ型形成的子实体均为畸形;Ⅳ、Ⅵ、Ⅶ型无子实体形成能力。菌丝生长速度依次为Ⅴ型>Ⅳ型>Ⅶ型>Ⅵ型>Ⅲ型>Ⅱ型>Ⅰ型,高产型菌落生长速度最慢,而低产型菌落菌丝徒长,表明菌落生长速度与子实体产量成负相关。优型菌落——Ⅰ型菌落子实体高产及高产保持代数最多的矿质元素配方为K3Mg2Ca4Mn3Zn2,其中Mn和K促进子实体高产,K、Mg、Ca有利于高产保持多代。抑制Ⅰ型菌落菌丝徒长的最佳配方为K3Mg2Ca2Mn3Zn2,其中Mn对其抑制作用最为显著。
To elucidate the relationship among mineral elements,the yield of fruit-body and growth rate of mycelia,effects of mineral elements on the fruit-body yield and growth rate of mycelia of different colony types of Cordyceps militaris in subculturing were investigated.L20,a good strain,was subcultured on different mineral elements media for twelve times with orthogonal experimental method.The results showed that there were seven colony types of L20 after subcultured,among which type Ⅰ,Ⅱ,Ⅲ and Ⅴ had ability to form fruit-body;however,type Ⅳ,Ⅵ and Ⅶ could not.Type Ⅰ performed the highest yield of fruit-body,type Ⅱ the lowest,and type Ⅲ in mid-level;while all the fruit-body produced by type Ⅴ were deformity.The growth rate of mycelia of different colony types were as follow：type Ⅴ type Ⅳ type Ⅶ type Ⅵ type Ⅲ type Ⅱ typeⅠ.The high-production colonies had slower growth rate of mycelia,while the low-production types had over growth of hyphae,indicating there was a negative correlation between the growth of mycelia and fruit-body yield.The formulation of mineral elements,which kept type Ⅰhigh yield and maintained more generations was K3Mg2Ca4Mn3Zn2,of which Mn,and K were able to produce high yield of fruit-body;K,Mg and Ca could maintain more generations to produce high yield.The best formulation which suppressed excessive growth rate of mycelia of type Ⅰ was K3Mg2Ca2Mn3Zn2,and Mn had the most significant effect.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第9期1331-1340,共10页
Microbiology China
基金
辽宁省攻关项目(No2006215001)
关键词
北虫草
矿质元素
子实体
继代培养
菌丝生长速度
正交试验
Cordyceps militaris
Mineral elements
Fruit-body
Subculturing
Growth rate of mycelia
Orthogonal experimental
