摘要
本研究选用了前列腺素类化合物(Prostaglandin E2(PGE2)、Prostaglandin F2α(PGF2α)、Prostaglandin D2(PGD2))和受体选择性激动剂(Butaprost、U-46619),用多道生理信号采集系统测定其对体外分离的奶牛输卵管峡部及壶腹部平滑肌收缩的影响,以此初步揭示受体的种类。结果发现,PGE2和Butaprost均抑制了奶牛输卵管峡部、壶腹部的自发性收缩运动,呈现明显的浓度依存性舒张反应;PGF2α在高浓度(1～10μmol/L)下,使峡部和壶腹部的自发性收缩运动有了显著增强;PGD2在低浓度时轻微抑制了壶腹部平滑肌的收缩,但在高浓度(1～10μmol/L)时加强了峡部和壶腹部平滑肌的收缩;U-46619从低浓度便引起了输卵管峡部和壶腹部平滑肌的强烈收缩。结果表明,EP4、EP2、FP、DP和TP受体同时存在于奶牛输卵管平滑肌上,能与内源性前列腺素类结合并参与调节输卵管平滑肌的自发性收缩。
To clarify the prostanoid receptors in bovine oviductal smooth muscle,the effects of prostaglandins（prostaglandin E2,PGE2;prostaglandin F2α,PGF2α;prostaglandin D2,PGD2） and receptor selective agonists（butaprost,U-46619） were examined on the bovine oviductal smooth muscle using physiological signals collection system in vitro.The results showed that PGE2 and butaprost concentration dependently inhibited tonic and spontaneous contraction of both the ampulla and the isthmus smooth muscle strips;PGF2α induced significantly contractile response in ampulla and isthmus smooth muscle isolated from bovine oviduct at high concentrations（1 to 10 μmol/L）;PGD2 inhibited the ampulla smooth muscle contraction at low concentrations,while at high concentrations（1 to 10 μmol/L） the ampulla and isthmus smooth muscle had been significantly contractile response;U-46619 induced potent contractile response in both the ampulla and the isthmus smooth muscle.These results suggested that EP4 or EP2,FP,DP,TP receptors were in the bovine oviductal smooth muscle,and the endogenous prostaglandins coupled these receptors contribute in regulation of oviductal smooth muscle contractility.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第9期118-123,共6页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古农业大学博士启动基金项目(K11648)
