摘要
以鸡冠花顶芽为外植体,采用不同激素组合对试管鸡冠花的芽诱导以及花诱导进行试验研究。结果表明:培养基MS+6-BA 0.6 mg/L+NAA 0.1 mg/L诱导的芽数量最多,而且芽生长表现较好,为最适芽诱导培养基;改良MS+6-BA 0.2 mg/L+NAA 0.01 mg/L+PP333 0.5 mg/L诱导的花大且都集中在主茎上,花型紧凑美观,为最适的试管苗开花培养基。在组培的基础上采用多效唑(PP333)调控花芽的形成,将为目前市场上试管苗开花的商品化研究提供数据资料。
Using the terminal buds of Celosia cristata as explants,the different concentration of plant hormone combinations on inducing the shoots differentiation and the flower buds differentiation were studied in this paper.The results were as follows：The optimal medium for shoot induction was Ms＋6-BA 0.6 mg/L＋NAA 0.1 mg/L which induced more normal shoots than other medium;the optimal medium for plantlet flowering was refined MS＋6-BA 0.2 mg/L＋NAA 0.01 mg/L＋PP333 0.5 mg/L which induced big and compact flowers on the main stems.The culture medium containing PP333 was good for controlling flower bud formation in the experiment,and the results supplied the research data for developing the test-tube flower in market.
关键词
鸡冠花
组织培养
试管内开花
Celosia cristata
tissue culture
in vitro flowering
